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Updated: Aug 19, 2025
Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
Published on: November 3, 2018
Neoplastic bone marrow invasion:rapid exclusion of hematological disease by flow cytometric routine panels
Maria Laura Bisegna1, Iole Cordone2, Nadia Peragine1
1Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
Insights
Multiparametric flow cytometry aids in identifying non-hematopoietic cells, crucial for diagnosing blood disorders. This technique helps exclude non-hematological origins, guiding appropriate patient management in hematology.
Area of Science:
- Immunology
- Hematology
- Cellular Biology
Background:
- Multiparametric flow cytometry (MFC) is vital for analyzing cellular populations in immunology and hematology.
- Non-hematopoietic cells can be encountered during routine immunophenotyping, presenting diagnostic challenges.
Purpose of the Study:
- To highlight the utility of MFC in identifying non-hematopoietic cells.
- To demonstrate MFC's role in excluding non-hematological origins in suspected blood disorders.
Main Methods:
- Utilized MFC with specific antibody panels (CD45, lineage antigens, CD56, CD117, CD138).
- Analyzed cases with clinical suspicion of hematological disease and unclear cytology.
Main Results:
- MFC successfully identified non-hematopoietic cells in 6 patients.
- The technique was crucial in excluding blood disorders, facilitating prompt diagnostic workups.
Conclusions:
- MFC is a powerful tool for differentiating non-hematopoietic cells in hematological investigations.
- This method accelerates accurate diagnosis and appropriate patient management when cytology is inconclusive.
Abstract:
Multiparametric flow cytometry is an extensively used technique to assess the presence of different cellular populations in immunology and hematology. During routine immunophenotyping analysis, it is not uncommon to face cells of non-hemopoietic origin, negative for CD45 and other myeloid, megakaryocytic, B and T lineage antigens and positive for at least one antibody among CD56, CD117 and CD138. If cytology cannot identify cell origin, especially in cases of unclear interpretation, the contribution of multiparametric flow cytometry analysis can be crucial. We report 6 patients with a clinical suspicion of hematological disease in which multiparametric flow cytometry was extremely useful to quickly exclude blood disorders in order to initiate patients to the most appropriate diagnostic process.
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