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Development of carbon nanoparticles-based soluble solid-phase immune sensor for the quantitative diagnosis of
Sulhee Kim1, Miyoung Koo2, Yukyung Tak3
1Department of Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Insights
A novel carbon nanoparticle (CNP) immunoassay simplifies quantitative immunodiagnosis. This method overcomes limitations like blood dilution and the hook effect, enabling rapid point-of-care diagnostics.
Area of Science:
- Biotechnology
- Nanotechnology
- Immunodiagnostics
Background:
- Quantitative immunodiagnosis is crucial for in vitro diagnostics.
- Existing methods often involve complex steps like blood dilution and mixing, risking errors such as the hook effect.
Purpose of the Study:
- To develop a simplified immunoassay using carbon nanoparticles (CNPs).
- To create a flexible, soluble solid-phase immune sensor for diverse in vitro diagnostic devices.
- To mitigate common issues in quantitative immunodiagnosis, including the hook effect.
Main Methods:
- Conjugation of antibodies to CNPs and integration with polymer materials to form a soluble solid-phase immune sensor.
- Development of a free-antibody system with dual calibration.
- Validation using canine C-reactive protein as the target immune analyte.
Main Results:
- The CNP-based immunoassay demonstrated high flexibility and solubility.
- The assay effectively mitigated the hook effect, performing excellently in a single step without pre-treatment.
- Dual calibration and free antibodies allowed for controlled detection ranges.
Conclusions:
- The developed CNP-based immunodiagnosis method offers a simplified, efficient, and reliable approach.
- This technology shows potential for advancing the commercialization of point-of-care immune biosensors.
- The assay eliminates the need for blood dilution and reaction mixing, reducing diagnostic errors.
Abstract:
Quantitative immunodiagnosis is one of the most commonly used methods for in vitro diagnostics. Various bioanalytical methods have been developed to quantitatively diagnose immune analytes; however, they require blood dilution pretreatment, reaction mixing, complicated experimental steps, and can cause diagnostic errors due to the hook effect. To address this issue, we introduced a simple immunoassay based on carbon nanoparticles (CNPs). The assay was designed to have high flexibility for use in various in vitro diagnostic devices by constructing a soluble solid-phase immune sensor with high solubility using antibody-conjugated CNPs and polymer materials. Excellent performance was achieved using a free-antibody system with dual calibration. To verify the performance of this method with high reliability, canine C-reactive protein was selected as the immune analyte. Interestingly, our method efficiently mitigated the hook effect with outstanding performance in a one-step reaction without blood dilution or reaction mixing. The detection range of the target can be effectively controlled using free antibodies. Therefore, our CNP-based immunodiagnosis method may advance the commercialization of point-of-care immune biosensors.
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