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Imaging Mass Cytometry in Immuno-Oncology
Tiziana Cotechini1, Oliver Jones2, Charles Colin Thomas Hindmarch3
1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. t.cotechini@queensu.ca.
Insights
Imaging mass cytometry (IMC) allows simultaneous detection of up to 50 markers in fixed tissues. This multiplex imaging technique provides spatially resolved insights into the tumor-immune microenvironment (TiME) for research.
Area of Science:
- Biomedical research
- Immunology
- Oncology
Background:
- Accurate profiling of the tumor-immune microenvironment (TiME) is crucial for understanding cancer biology and developing effective therapies.
- Simultaneous detection of multiple proteins within the TiME is essential for comprehensive analysis.
Purpose of the Study:
- To provide an overview of the imaging mass cytometry (IMC) workflow for in situ profiling of the TiME.
- To highlight the potential of IMC in pre-clinical and clinical research.
Main Methods:
- Overview of the IMC workflow, including sample fixation, multiplex panel design, tissue staining, and data analysis.
- Utilizing the Hyperion™ imaging system for multiplex imaging.
Main Results:
- IMC enables detection of up to 50 markers at subcellular resolution on fixed tissues.
- Provides spatially resolved information critical for understanding the TiME.
Conclusions:
- IMC is a powerful multiplex imaging modality for detailed analysis of the TiME.
- The presented workflow facilitates the application of IMC in both pre-clinical and clinical settings.
Abstract:
In situ profiling of the tumor-immune microenvironment (TiME) requires the ability to co-localize and detect multiple proteins simultaneously. Imaging mass cytometry (IMC), using the Hyperion™ imaging system is a novel multiplex imaging modality that currently enables detection of up to 50 markers on fixed tissues at subcellular resolution and thus has the potential to inform both pre-clinical and clinical research by providing investigators with spatially resolved information about the TiME. Here we provide an overview of the IMC workflow from sample fixation to analysis, with a focus on multiplex panel design and tissue staining.
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