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Updated: Aug 15, 2025
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
Published on: June 7, 2018
Analyzing the Tumor-Immune Microenvironment by Flow Cytometry
François Santinon1, Yoon Kow Young2, Sonia V Del Rincón2,3
1Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada. francoissantinon@gmail.com.
Insights
This study details flow cytometry methods for analyzing the tumor-immune microenvironment. It provides a protocol for reproducible cell staining and data analysis in lymphoid tissues and solid tumors.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Flow cytometry is crucial for dissecting the complex tumor-immune microenvironment.
- Accurate quantification and identification of diverse cell populations within heterogeneous samples are vital for cancer research.
Purpose of the Study:
- To provide an overview of flow cytometry instrumentation.
- To discuss considerations and steps for developing reproducible flow cytometry staining panels.
- To present an updated protocol for lymphoid tissue and solid tumor-infiltrating leukocyte analysis.
Main Methods:
- Review of flow cytometry instrumentation principles.
- Detailed steps for building a reproducible flow cytometry staining panel.
- Presentation of an updated protocol for analyzing lymphoid tissues and solid tumors.
- Exemplification of flow cytometry data analysis techniques.
Main Results:
- Established guidelines for flow cytometry panel design.
- Provided a validated protocol for immune cell profiling in tumor tissues.
- Demonstrated a workflow for flow cytometry data analysis.
Conclusions:
- Flow cytometry is an indispensable technique for tumor-immune microenvironment research.
- Reproducible panel design and standardized protocols enhance data reliability.
- The presented methods facilitate comprehensive immune cell analysis in cancer samples.
Abstract:
Flow cytometry is an essential tool for studying the tumor-immune microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. This chapter provides an overview of the flow cytometry instrumentation and a discussion of the appropriate considerations and steps in building a reproducible flow cytometry staining panel. We present an updated lymphoid tissue and solid tumor-infiltrating leucocyte flow cytometry staining protocol and an example of flow cytometry data analysis.
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