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Updated: Aug 15, 2025
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
Published on: September 26, 2019
Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
Keltouma Benmeziane1, Benoit Delache1, Sébastien Langlois1
1Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), 92265 Fontenay-aux-Roses and Le Kremlin-Bicêtre, France.
Insights
This study presents an optimized protocol for isolating high yields of viable intestinal lamina propria mononuclear cells (LPMCs) from human and nonhuman primate tissues. The method ensures repeatable and scalable cell isolation for further immune cell research.
Area of Science:
- Immunology
- Cell Biology
- Gastroenterology
Background:
- Characterizing immune cells in tissues is vital for understanding health and disease.
- Intestinal lamina propria mononuclear cells (LPMCs) play key roles in gut immunity.
Purpose of the Study:
- To provide an optimized protocol for high-yield isolation of viable LPMCs.
- To detail methods for characterizing mononuclear phagocyte (MP) subtypes within LPMCs.
Main Methods:
- Optimized protocol for intestinal tissue collection from humans and nonhuman primates.
- Mechanical disruption and enzymatic digestion for cell isolation.
- Flow cytometry analysis for mononuclear phagocyte (MP) subtype characterization.
Main Results:
- Achieved high yields of viable intestinal lamina propria mononuclear cells (LPMCs).
- Successfully characterized mononuclear phagocyte (MP) subtypes.
- Protocol demonstrated repeatability and scalability.
Conclusions:
- The optimized protocol facilitates robust isolation of viable LPMCs.
- Enables detailed characterization of immune cell populations, particularly MPs.
- Supports downstream applications in immunological and disease research.
Abstract:
Isolation of viable immune cells from tissues is critically important to characterize cellular and molecular processes during homeostasis and disease. Here, we provide an optimized protocol to achieve high yields of viable intestinal lamina propria mononuclear cells (LPMCs). We describe steps for intestinal tissue collection from humans and nonhuman primates, followed by mechanical disruption and enzymatic digestion. Furthermore, we detail characterization of the mononuclear phagocyte (MP) subtypes by flow cytometry analysis. The protocol is repeatable and scalable for downstream applications. For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022).
