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CPHEN-15: Comprehensive phenotyping of human peripheral blood helper-ILCs by flow cytometry
Genny Del Zotto1, Paola Vacca2, Lorenzo Moretta2
1Istituto Giannina Gaslini, IRCCS, Genoa, Italy.
Insights
This review offers guidelines for studying human peripheral blood helper innate lymphoid cells (ILCs). It details markers for analyzing these immune cells
Area of Science:
- Immunology
- Cell Biology
Background:
- Innate lymphoid cells (ILCs) are crucial for immune defense and tissue homeostasis.
- Helper-ILCs, including ILC1, ILC2, and ILC3 subsets, mirror T-helper cell functions.
- While primarily tissue-resident, helper ILCs circulate in peripheral blood (PB).
Approach:
- This review focuses on analyzing human peripheral blood (PB) helper ILCs.
- It provides guidelines and a marker list for flow cytometry studies.
- The aim is to facilitate comparison between pathological and physiological conditions.
Key Points:
- Helper-ILCs contribute to host defense, homeostasis, and repair via cytokine secretion.
- Analyzing circulating helper ILCs in human PB is important for clinical research.
- Flow cytometry is a key method for assessing PB helper ILC phenotype and function.
Conclusions:
- This work offers practical guidance for researchers studying human helper ILCs in peripheral blood.
- The provided marker list aids in detailed phenotypical and functional analysis.
- Understanding circulating helper ILCs is vital for advancing immunology and clinical diagnostics.
Abstract:
Innate lymphoid cells (ILCs) comprise cytotoxic NK cells and helper-ILCs, which are further divided in ILC1, ILC2, and ILC3. Helper-ILCs mirror the effector functions of helper T-cell subsets and contribute to host immune defense, tissue homeostasis and repair through cytokine secretion. Although they are mainly tissue-resident, helper ILCs are also found in the peripheral blood (PB). In the human setting, it may be needed to analyze circulating helper ILCs to compare pathological to physiological conditions. In this review, we provide simple guidelines and a list of markers useful to study human PB helper ILCs phenotype and function by flow cytometry.
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