Related Experiment Video

Updated: Aug 8, 2025

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.8K

Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application

Patricia J T A Groenen1,2, Michiel van den Brand1,3, Leonie I Kroeze1,2

  • 1Department of Pathology, Radboud University Medical Center, Nijmegen, Netherlands.

Frontiers in Oncology
|February 27, 2023
PubMed

Insights

Next-generation sequencing (NGS) offers a more sensitive method for detecting immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements. This advanced clonality testing aids in diagnosing lymphomas and other conditions.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Clonality assessment of immunoglobulin (IG) and T-cell receptor (TR) genes is crucial for diagnosing B-cell and T-cell lymphomas.
  • Conventional fragment analysis methods for clonality testing have limitations in sensitivity and precision.

Purpose of the Study:

  • To develop and validate a next-generation sequencing (NGS)-based clonality assay for IG and TR gene rearrangements.
  • To enhance sensitivity and precision in clone detection and comparison compared to traditional methods.

Main Methods:

  • Development and validation of an NGS-based assay for IG heavy and kappa light chain, and TR gene rearrangements.
  • Application of the assay to formalin-fixed and paraffin-embedded tissues.

Main Results:

  • The NGS-based assay provides more sensitive detection of lymphocyte clones.
  • It allows for more precise comparison of clones than conventional fragment analysis.

Conclusions:

  • NGS-based clonality testing is a powerful tool for diagnosing lymphomas.
  • Potential applications include site-specific lymphoproliferations, immunodeficiency, autoimmune diseases, and monitoring primary and relapsed lymphomas.

Keywords:
NGST-cell receptorclonality assessmentgene rearrangementimmunoglobulin Epathology

More Related Videos

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.1K
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.5K

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
66
Karyotyping01:17

Karyotyping

Overview
62.0K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
1.8K