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Quantitatively detecting Candida albicans enolase1 with a one-step double monoclonal antibody sandwich ELISA assay
Jingzi Piao1, Ning Li2, Lina Zhang3
1College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China.
Insights
A new enzyme-linked immunosorbent assay (ELISA) detects Candida albicans enolase1 (CaEno1) for invasive candidiasis (IC) diagnosis. This CaEno1 assay shows improved diagnostic efficiency over existing methods in a rabbit model.
Area of Science:
- Medical Microbiology
- Immunology
- Biotechnology
Background:
- Invasive candidiasis (IC) poses a significant threat to hospitalized patients, especially the critically ill.
- Current laboratory diagnostic techniques for IC are insufficient, complicating disease management.
- Early and accurate diagnosis is crucial for effective treatment of IC.
Purpose of the Study:
- To develop and validate a novel diagnostic assay for invasive candidiasis.
- To quantitatively detect Candida albicans enolase1 (CaEno1) as a potential biomarker for IC.
- To evaluate the diagnostic efficiency of the developed assay compared to existing methods.
Main Methods:
- Development of a one-step double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA).
- Utilized specific monoclonal antibodies (mAbs) for the detection of CaEno1.
- Validated the assay using a rabbit model of systemic candidiasis and compared results with (1,3)-β-D-glucan detection and blood culture.
Main Results:
- The developed DAS-ELISA demonstrated sensitivity, reliability, and feasibility.
- CaEno1 detection assay showed superior diagnostic efficiency compared to (1,3)-β-D-glucan and blood culture in the rabbit model.
- CaEno1 was detected transiently and at low levels in infected rabbit plasma.
Conclusions:
- The CaEno1 detection assay is a promising tool for diagnosing invasive candidiasis.
- Combining CaEno1 antigen and IgG antibody detection may enhance diagnostic accuracy.
- Further technical development is needed to improve the detection limit for broader clinical application.
Abstract:
Invasive candidiasis (IC) is often a cause of severe concern for the hospitalized patients, particularly those who are critically sick. However management of this disease is challenging due to a lack of effective laboratory diagnostic techniques. Hence, we have developed a one-step double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using a pair of specific monoclonal antibodies (mAbs) for the quantitative detection of Candida albicans enolase1 (CaEno1), which is considered as an important diagnostic biomarker for IC. The diagnostic efficiency of the DAS-ELISA was evaluated by using a rabbit model of systemic candidiasis and compared with other assays. The method validation results demonstrated that the developed method was sensitive, reliable, and feasible. The findings of the rabbit model plasma analysis indicated that the diagnostic efficiency of the CaEno1 detection assay was better in comparison to the (1,3)-β-D-glucan detection and blood culture. CaEno1 is present in the blood of infected rabbits for a brief period and at relatively low levels and thus the combination of CaEno1 antigen and IgG antibodies detection could aid to increase diagnostic efficiency. However, to improve the clinical application of CaEno1 detection in the future, efforts should be made to increase the detection limit of the test by promoting technical developments and by optimizing the protocol for the clinical serial determinations.
