Related Experiment Video

Updated: Jul 22, 2025

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures
04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Published on: November 22, 2024

360

Crosstalk between TRPV1 and immune regulation in Fuchs endothelial corneal dystrophy

Yuchen Cai1, Jin Chen1, Hao Sun1

  • 1Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China.

Clinical Immunology (Orlando, Fla.)
|July 23, 2023
PubMed

Insights

Fuchs endothelial corneal dystrophy (FECD) involves Transient Receptor Potential Vanilloid 1 (TRPV1) and immune cells. This study identifies TRPV1-related genes and pathways, suggesting a new therapeutic target for FECD.

Area of Science:

  • Ophthalmology
  • Immunology
  • Molecular Biology

Background:

  • Fuchs endothelial corneal dystrophy (FECD) is a primary cause of corneal transplantation globally.
  • The role of Transient Receptor Potential Vanilloid subtype 1 (TRPV1) and immune regulation in FECD pathogenesis remains unclear.

Purpose of the Study:

  • To investigate the involvement of TRPV1 and associated immune responses in FECD.
  • To identify key genes and pathways linking TRPV1 to FECD.

Main Methods:

  • Utilized an in vitro FECD model with H₂O₂ induction.
  • Analyzed gene expression microarray datasets (GSE142538, GSE112039, E-GEAD-399 & 564).
  • Performed functional analyses on TRPV1-related differentially expressed genes (DEGs) and in vitro validation.

Main Results:

  • TRPV1 was significantly upregulated in the in vitro FECD model.
  • A negative correlation was observed between TRPV1 expression and immune cells, particularly regulatory T cells (Tregs).
  • Identified four core TRPV1-related genes (MAPK14, GNB1, GNAQ, ARRB2) and implicated TRP-regulated calcium transport and inflammatory pathways.

Conclusions:

  • TRPV1 and immune regulation exhibit potential crosstalk in FECD pathogenesis.
  • The identified biomarkers and pathways offer a novel framework for FECD research and therapeutic development.

Keywords:
Fuchs endothelial corneal dystrophyGene therapyImmune landscapeRegulatory T cellTransient receptor potential vanilloid 1

More Related Videos

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition09:05

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

Published on: August 20, 2016

8.0K
Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation09:03

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Published on: November 17, 2014

9.8K

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K