Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging
David Alejandro Bejarano1, Andreas Schlitzer2
1Quantitative Systems Biology, LIMES Institute, University of Bonn, Bonn, Germany.
Insights
This study uses Co-Detection by Indexing (CODEX) to reveal the diverse phenotypes and locations of tissue-resident macrophages. The findings offer insights into macrophage heterogeneity and their interactions within various organs.
Area of Science:
- Immunology
- Cell Biology
- Spatial Biology
Background:
- Macrophages exhibit significant phenotypic diversity and plasticity.
- This heterogeneity is shaped by the tissue microenvironment.
Purpose of the Study:
- To visualize phenotypic and spatial heterogeneity of murine tissue-resident macrophages.
- To characterize macrophage cellular microenvironments and interactions.
Main Methods:
- Utilized Co-Detection by Indexing (CODEX), a multiplexed imaging technique.
- Enabled simultaneous detection of multiple cellular markers for single-cell spatial identification.
- Applied a computational pipeline for microenvironment characterization.
Main Results:
- Visualized phenotypic and spatial heterogeneity of macrophages across multiple murine organs.
- Characterized distinct cellular microenvironments and cell-cell interactions.
- Identified tissue-resident hematopoietic and non-hematopoietic cells with spatial context.
Conclusions:
- CODEX is effective for mapping macrophage heterogeneity in situ.
- Understanding spatial interactions is crucial for macrophage function.
- This approach provides a framework for studying tissue-specific immune cell niches.
Abstract:
Macrophages display a high degree of phenotypic diversity and plasticity, which is influenced by their location within the tissue microenvironment. Co-Detection by Indexing (CODEX), a multiplexed imaging technique, allows the simultaneous detection of multiple membrane and cellular markers that enable the accurate identification of tissue-resident hematopoietic and non-hematopoietic cells, while conferring spatial information at a single-cell level. Here we describe the use of CODEX to visualize the phenotypic and spatial heterogeneity of murine tissue-resident macrophages in several organs, and a pipeline to characterize their cellular microenvironments and interactions.
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