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Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-γ1(S1248)

Chang Liu1, Weifeng Yuan2, Hao Yang1

  • 1College of Life Sciences, Hebei University , Baoding, China.

Microbiology Spectrum
|September 1, 2023
PubMed

Insights

Bovine herpesvirus 1 infection activates phospholipase C gamma 1 (PLC-γ1) signaling, leading to its accumulation in the Golgi apparatus. This activated PLC-γ1 escorts viral particles for release, revealing a novel mechanism in virus replication.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Phospholipase C gamma 1 (PLC-γ1) is known to localize in various cellular compartments, including the cytosol, plasma membrane, and nucleus.
  • Bovine herpesvirus 1 (BoHV-1) productive infection is associated with increased levels of phosphorylated PLC-γ1 at Ser1248 (p-PLC-γ1(S1248)), which supports viral replication.

Purpose of the Study:

  • To investigate the subcellular localization of activated p-PLC-γ1(S1248) during BoHV-1 infection.
  • To elucidate the role of p-PLC-γ1(S1248) in the BoHV-1 life cycle, particularly in relation to viral particle trafficking.

Main Methods:

  • Confocal microscopy to visualize p-PLC-γ1(S1248) localization in infected cells.
  • Coimmunoprecipitation assays to identify proteins interacting with Golgi-localized p-PLC-γ1(S1248).
  • Treatment with a PLC-γ1-specific inhibitor (U73122) to assess the functional role of PLC-γ1 signaling in viral egress.

Main Results:

  • Activated p-PLC-γ1(S1248) was found to accumulate in the Golgi apparatus during later stages of BoHV-1 infection.
  • Golgi-associated p-PLC-γ1(S1248) specifically binds to the viral protein gD, but not gC.
  • Inhibition of PLC-γ1 signaling led to the retention of viral protein gD within the Golgi apparatus, suggesting impaired virion egress.

Conclusions:

  • This study identifies the Golgi apparatus as a novel cellular compartment containing activated p-PLC-γ1(S1248) during BoHV-1 infection.
  • Activated p-PLC-γ1(S1248) is associated with both intracellular and released virions, and its interaction with gD suggests a role in viral particle transport.
  • PLC-γ1 signaling appears to facilitate the trafficking of progeny virions from the Golgi apparatus, representing a new mechanism in BoHV-1 productive infection.
Abstract

Keywords:
Golgi apparatusbovine herpesvirus 1gDphospholipase C-γ1

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