Intravital microscopy visualizes innate immune crosstalk and function in tissue microenvironment

Area of Science:

• Immunology
• Cell Biology
• Microscopy

Background:

• Intravital microscopy (IVM) has seen significant progress in studying myeloid cells.
• Advances in fluorescent probes and reporter mice facilitate IVM applications.
• IVM enables direct observation of cellular behavior in native tissue environments.

Purpose of the Study:

• To review current studies on the dynamic interaction and function of innate immune cells using IVM.
• To highlight advances in data analysis, including artificial intelligence (AI) algorithms.
• To discuss the future prospects of IVM in innate immune cell research.

Main Methods:

• Review of existing literature on intravital microscopy of myeloid and innate immune cells.
• Analysis of studies employing fluorescent probes and reporter mice.
• Examination of emerging AI-based algorithms for data analysis in IVM.

Main Results:

• IVM is crucial for understanding myeloid cell dynamics, heterogeneity, plasticity, and communication.
• Current research focuses on innate immune cell interactions visualized through IVM.
• AI algorithms are improving the analysis of complex IVM data.

Conclusions:

• IVM is a powerful tool for investigating innate immune cell behavior in vivo.
• Continued development of probes, reporter systems, and AI will further advance the field.
• Future research will benefit from enhanced visualization and analytical capabilities in IVM.