Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems-A Scoping Review

Area of Science:

• Immunology
• Physiology
• Microscopy

Background:

• Intravital microscopy (IVM) enables real-time observation of biological processes within a living organism.
• Ex vivo and in vitro models lack the physiological context crucial for understanding complex cellular interactions.
• IVM is vital for studying immune responses, particularly leukocyte-endothelial interactions in the microcirculation.

Purpose of the Study:

• To provide an overview of intravital microscopy (IVM) applications in studying lipopolysaccharide (LPS)-induced inflammation.
• To summarize findings from IVM studies examining inflammatory responses in various organ systems.
• To highlight the utility of IVM in diverse pathological models.

Main Methods:

• Review of existing literature on IVM studies involving LPS-induced inflammation.
• Analysis of IVM imaging techniques for visualizing cellular dynamics in vivo.
• Synthesis of data from studies conducted in brain, intestines, bladder, and lung models.

Main Results:

• IVM effectively visualizes acute inflammatory changes induced by LPS in multiple organs.
• Leukocyte recruitment and microcirculatory alterations are key observable phenomena.
• The technique allows for detailed characterization of immune cell behavior during inflammation.

Conclusions:

• Intravital microscopy is an indispensable tool for investigating LPS-induced inflammation in vivo.
• IVM provides critical insights into immune cell dynamics and microvascular responses.
• The reviewed studies demonstrate the broad applicability of IVM across different organ systems for inflammatory research.