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Rapid Immunohistochemistry Based on Ultrasonic Thermal Steam Heating for Improvement of Intraoperative Diagnosis
Guangyin Yu1, Lili Tao, Mengying Liao
1Department of Pathology, Peking University Shenzhen Hospital, Futian, Shenzhen, Guangdong, P.R. China.
Insights
Rapid immunohistochemistry (RIHC) using ultrasonic steam heating significantly improves intraoperative frozen section diagnosis accuracy. This cost-effective method is practical for pathology departments, aiding differential diagnosis.
Area of Science:
- Pathology
- Histology
- Medical Diagnostics
Background:
- Intraoperative frozen section diagnosis is critical for surgical decision-making.
- Challenges exist in achieving rapid and accurate diagnoses during surgery.
- Immunohistochemistry (IHC) can enhance diagnostic accuracy but is often time-consuming.
Purpose of the Study:
- To evaluate the efficacy of rapid immunohistochemistry (RIHC) utilizing ultrasonic thermal steam heating.
- To assess RIHC's role in improving diagnostic accuracy for intraoperative frozen sections.
- To recommend optimal RIHC antibody panels for differential diagnosis.
Main Methods:
- RIHC employing ultrasonic thermal steam heating was applied to challenging intraoperative frozen section cases.
- Ninety-three surgical specimens underwent RIHC examination.
- RIHC slide diagnoses were compared with final paraffin-embedded diagnoses to calculate discordance rates.
Main Results:
- RIHC proved helpful in 85 out of 93 cases (91% utility rate).
- A total of 58 antibodies were tested 276 times, with 19 antibodies showing ineffectiveness in 25 instances.
- Fifteen RIHC antibody panels were recommended based on staining stability and frequency of use.
Conclusions:
- Ultrasonic thermal steam heating RIHC is a practical, convenient, and cost-effective technique.
- The improved RIHC method is suitable for routine use in any pathology laboratory.
- RIHC serves as a valuable auxiliary tool for enhancing the accuracy of intraoperative rapid pathologic diagnoses.
Objectives:
To evaluate the role of rapid immunohistochemistry (RIHC) based on ultrasonic thermal steam heating in improving diagnostic accuracy of intraoperative frozen section diagnosis and to recommend RIHC antibody panels for pathologic differential diagnosis.
Materials And Methods:
RIHC based on ultrasonic thermal steam heating was tested for intraoperative frozen diagnosis with difficulty in diagnosis, and all slides were reviewed and compared with the final diagnosis. Ninety-three cases of surgical specimens involving RIHC examination were studied. Discordance rates with paraffin immunohistochemistry were calculated.
Results:
In 93 cases where RIHC was performed, 85 cases (91%) were proven to be helpful for the diagnosis. A total of 58 antibodies were used for RIHC 276 times, of which 19 antibodies were not effective 25 times. Fifteen RIHC antibody panels are recommended based on staining stability and utilization frequency.
Conclusion:
After improving the staining method, ultrasonic thermal steam heating RIHC is practical, convenient, and cost-effective, making it suitable for use in any pathology department with routine immunohistochemistry reagents. It plays an important auxiliary role in improving the accuracy of intraoperative rapid pathologic diagnosis.
