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Updated: Jun 27, 2026
Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
Immunophenotyping of Leukocytes in Amniotic Fluid
Yi Xu1, Derek Miller2, Jose Galaz3
1Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
Insights
This study details a method for immunophenotyping amniotic fluid leukocytes. This technique provides insights into immune activity within the amniotic cavity.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Leukocyte characterization is crucial for understanding immune responses.
- Amniotic fluid contains immune cells that play a role in fetal development and health.
- Current methods for analyzing amniotic fluid immune cells are limited.
Purpose of the Study:
- To establish a comprehensive protocol for the immunophenotyping of amniotic fluid.
- To enable detailed analysis of immune cell populations within the amniotic cavity.
- To provide a foundation for studying immune processes in the amniotic environment.
Main Methods:
- Detailed protocol for amniotic fluid cell count determination and processing.
- Application of viability, extracellular, and intracellular/intranuclear antibody staining.
- Flow cytometry acquisition for high-dimensional immune cell analysis.
Main Results:
- A validated procedure for immunophenotyping amniotic fluid leukocytes.
- Demonstration of deep characterization of immune cells in biological samples.
- Establishment of a method to gain information on immune processes in the amniotic cavity.
Conclusions:
- The described immunophenotyping protocol is effective for analyzing amniotic fluid.
- This method offers valuable insights into the immune status of the amniotic cavity.
- The protocol facilitates further research into fetal immunology and related conditions.
Abstract:
Immunophenotyping allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the immunophenotyping of amniotic fluid, which can provide information into the immune processes taking place in the amniotic cavity. The protocol describes amniotic fluid cell count determination, processing, and the use of viability, extracellular antibody, and intracellular/intranuclear antibody staining prior to flow cytometer acquisition.
