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Updated: Jun 29, 2025
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
Comprehensive Immunophenotyping by Polychromatic Cytometry
Takuto Nogimori1, Takuya Yamamoto2
1Laboratory of Precision Immunology, Center for Intractable Diseases and ImmunoGenomics, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Insights
This study introduces a new multicolor flow cytometry panel for comprehensive immune cell analysis. The panel aids in understanding immune responses to aging, infections, and vaccines by assessing T and B cell characteristics and activation states.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- The human immune system relies on complex interactions between diverse innate and adaptive immune cells to combat pathogens.
- Multicolor flow cytometry is a key technique for analyzing immune cell populations, offering high-dimensional insights into cellular heterogeneity and function.
- Understanding immune cell dynamics is crucial for disease pathogenesis research and therapeutic development.
Purpose of the Study:
- To develop and validate a novel immunophenotyping panel for simultaneous evaluation of T and B cell characteristics.
- To incorporate markers for immune cell activation (co-stimulatory molecules) and exhaustion (inhibitory checkpoint molecules).
- To enable tracking of immune status changes related to aging, environmental factors, infections, and vaccination.
Main Methods:
- Development of a multicolor flow cytometry panel for simultaneous T and B cell immunophenotyping.
- Inclusion of specific markers for co-stimulatory and inhibitory checkpoint molecules to assess immune cell activation and exhaustion.
- Application of the panel to analyze immune cell populations in various physiological and pathological contexts.
Main Results:
- The new panel allows for simultaneous, high-dimensional analysis of T and B cell populations.
- It effectively tracks immune status changes influenced by aging, environmental factors, pathogen infections, and vaccine administration.
- The panel provides insights into immune cell activation and exhaustion states, correlating with disease pathophysiology and vaccine efficacy.
Conclusions:
- This novel immunophenotyping panel enhances the understanding of human immune responses.
- It offers a versatile tool for research in immunology, infectious diseases, aging, and vaccine development.
- The findings support the development of targeted therapeutic strategies and improved vaccine efficacy assessments.
Abstract:
The human immune system comprises a diverse array of cells involved in innate and adaptive immunity, and these immune cells coordinate immune responses against pathogens through intricate interactions. Multicolor flow cytometry is a powerful technique for qualitatively and quantitatively measuring the characteristics of immune cells, offering advantages, such as high-dimensional analysis, elucidation of cellular heterogeneity, understanding of pathogenesis, development of therapeutic strategies, and platform flexibility. Here, we demonstrate a new immunophenotyping panel that allows simultaneous evaluation of the characteristics of T and B cells. This panel enables tracking of changes in the immune status due to aging, environmental factors, pathogen infections, and vaccine administration. Additionally, it includes co-stimulatory molecules for assessing the activation state of immune cells and inhibitory checkpoint molecules for evaluating exhaustion status, thereby providing valuable insights into the features of human immune responses. These analyses contribute to understanding the pathophysiology of diseases and developing therapeutic strategies while offering crucial information for assessing the correlation of symptoms with infections and evaluating the efficacy of vaccines.
