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Updated: Sep 5, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
A new antigen test device for rapid influenza A and B detection
Hanan Rohana1, Guy Marmur2, Maya Azrad1
1Clinical Microbiology Laboratory, Tzafon Medical Center, Poriya, Israel.
Insights
The LabOn-Time™ Influenza A + B Rapid test shows 75.7% sensitivity and 100% specificity for detecting influenza. This rapid test aids in the early detection of influenza A/B infections.
Area of Science:
- Clinical diagnostics
- Virology
- Infectious diseases
Background:
- Accurate and rapid influenza detection is crucial for patient management.
- Existing diagnostic methods vary in speed, sensitivity, and specificity.
Purpose of the Study:
- To evaluate the diagnostic performance of the LabOn-Time™ Influenza A + B Rapid test.
- To compare the rapid test against Real-time polymerase chain reaction (RT-PCR) for influenza A/B detection.
Main Methods:
- 183 nasopharyngeal samples from patients with influenza-like symptoms were tested.
- LabOn-Time™ Influenza A + B Rapid test was compared with RT-PCR.
- Sensitivity, specificity, and odds ratios for false negatives were calculated.
Main Results:
- The LabOn-Time™ test demonstrated 75.7% sensitivity and 100% specificity compared to RT-PCR.
- False negatives were associated with higher RT-PCR cycle thresholds (≥20) and earlier symptom onset.
- Positive cases reported a significantly higher mean number of symptoms.
Conclusions:
- The LabOn-Time™ device is a simple and intuitive tool for influenza A/B detection.
- This rapid test can significantly contribute to the early diagnosis of influenza infections.
Introduction:
Tests for detection of influenza must demonstrate high sensitivity and specificity, affordability, and rapidness.
Methods:
This study aimed to evaluate the performance of the LabOn-Time™ Influenza A + B Rapid test device (BMT Diagnostics, Ltd), as compared to Real-time polymerase chain reaction (RT-PCR), in identifying influenza A/B among 183 nasopharyngeal samples collected between February and April 2023 from patients with Influenza-like symptoms.
Results:
Out of 70 participants with a positive RT-PCR result, 53 (75.7 %) had a positive LabOn-Time result. The LabOn-Time kit had a sensitivity of 75.7 % and specificity of 100 %. The odds ratio for showing a false negative LabOn-Time result for influenza B, compared to influenza A was 5.24 (95%CI: 1.35-20.31). All false negative LabOn-Time samples had a RT-PCT cycle threshold ≥20. Mean time from symptom onset was significantly lower in the false negative LabOn-Time cases compared to the positive cases (36 ± 15.3 vs. 42.6 ± 10.1, respectively). The mean number of symptoms reported per patient was significantly higher in positive compared to negative LabOn-Time cases (2.5 ± 0.5 vs. 1.9 ± 0.4, p < 0.001).
Conclusions:
The LabOn-Time device, which is very simple and intuitive to operate, could significantly contribute to early detection of influenza A/B infection.
