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Iridium oxide-modified reference screen-printed electrodes for point-of-care portable electrochemical cortisol

Tong Ji1, Wen Ye1, Weiyu Xiao1

  • 1Xi'an Jiaotong-Liverpool University, School of Science, Department of Chemistry, No. 111 Ren'ai Road, Suzhou Industrial Park, Suzhou, People's Republic of China.

Talanta
|August 31, 2024
PubMed

Insights

This study developed a novel electrochemical biosensor for sensitive cortisol detection. The new sensor utilizes anti-cortisol antibodies and iridium oxide for improved accuracy in stress biomarker measurement.

Area of Science:

  • Electrochemistry
  • Biomarker Detection
  • Biosensor Technology

Background:

  • Cortisol is a key stress biomarker.
  • Accurate measurement of cortisol is crucial for health monitoring.
  • Existing detection methods may lack sensitivity or selectivity.

Purpose of the Study:

  • To develop a selective and sensitive electrochemical immunosensor for cortisol detection.
  • To immobilize anti-cortisol antibodies on gold electrodes for biosensor fabrication.
  • To investigate the use of iridium oxide as a reference electrode for improved sensor performance.

Main Methods:

  • Fabrication of an electrochemical bio-immunosensor using anti-cortisol antibodies covalently bonded to gold electrodes.
  • Anodic electrodeposition of Iridium Oxide (IrOx) as a reference electrode on a screen-printed electrode.
  • Utilizing Electrochemical Impedance Spectrometry (EIS) to correlate sensor response with cortisol concentration.

Main Results:

  • A linear correlation was established between charge transfer resistance (Rct) and the logarithm of cortisol concentration (1 ng/mL to 1 mg/mL).
  • Achieved a limit of detection of 11.85 pg/mL (32.69 pM) for cortisol.
  • Iridium oxide modification significantly enhanced the reproducibility of the screen-printed electrode.

Conclusions:

  • The developed electrochemical immunosensor offers a reliable and sensitive method for cortisol measurement.
  • The integration of IrOx reference electrode improves sensor stability and reproducibility.
  • This biosensing system holds potential for accurate stress biomarker analysis.

Keywords:
CortisolElectrochemicalElectrochemical impedance spectrometry (EIS)Screen-printed electrodeStress

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