Related Experiment Video
Updated: Jun 13, 2025
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
Published on: August 11, 2018
Analysis of B cell proliferation in response to in vitro stimulation in patients with CVID
Velásquez-Ortiz Ma Guadalupe1, Saldaña-Solano Abigail J2, O'Farrill-Romanillos Patricia3
1Posgrado en Ciencias Biológicas UNAM, Ciudad Universitaria, México.
Insights
Common variable immunodeficiency (CVID) impairs B cell antibody production. Patient B cell proliferation defects vary based on memory B cell levels and the specific immune stimulus used.
Area of Science:
- Immunology
- Cell Biology
- Medical Research
Background:
- Common variable immunodeficiency (CVID) is a heterogeneous disorder.
- It is characterized by defective antibody production and impaired B cell differentiation.
- B cell proliferation is crucial for antibody synthesis, with T-cell-dependent and T-cell-independent responses.
Purpose of the Study:
- To investigate B cell proliferation capacity in CVID patients.
- To compare T-cell-dependent and T-cell-independent responses in CVID.
- To correlate B cell proliferation with patient B cell phenotypes.
Main Methods:
- Studied 23 CVID patients and 14 healthy donors.
- Categorized patients based on memory B cell percentage.
- Utilized in vitro CFSE dilution assay for B cell proliferation assessment.
Main Results:
- Reduced memory B cells correlated with increased Tfh cells and low proliferation to PKW, CpG, and SAC stimuli.
- Normal memory B cells showed high IL-21R expression and low proliferation to CpG, IL-21, CD40L, and anti-IgM stimuli.
- Proliferation defects were stimulus- and phenotype-dependent.
Conclusions:
- Defective B cell proliferation in CVID is influenced by stimulus type and patient phenotype.
- Further research is needed to elucidate CVID disease mechanisms.
- Identifying genetic defects associated with CVID is a future goal.
Background:
Common variable immunodeficiency (CVID) is a heterogeneous disorder characterized by defective antibody production and impaired differentiation of B cells. B cell proliferation is an essential step for antibody synthesis. Depending on the nature of the stimulus, their response may be either T-cell-dependent or T-cell-independent.
Methods:
We studied 23 CVID patients and 14 healthy donors (HD). The patients were categorized based on their percentage of memory B cells. In addition to standard immunophenotyping of circulating human B and T cell subsets, an in vitro CFSE dilution assay was used to assess the proliferative capacity of B cells and to compare the activation of the T cell-dependent and T cell-independent response among the patients.
Results:
Patients with a reduction in memory B cells exhibited an increase in follicular T cells (Tfh) and showed low proliferation in response to PKW, CpG, and SAC stimuli (Condition II) (p= 0.0073). In contrast, patients with a normal percentage of memory B cells showed a high expression of IL-21R and low proliferation in response to CPG (Condition III); IL-21, CD40L, and anti-IgM (Condition IV) stimuli (p= 0.0163 and p = 0.0475, respectively).
Conclusion:
Defective proliferation in patients depends on the type of stimulus used and the phenotypic characteristics of the patients. Further studies are necessary to understand the disease mechanisms, which may guide us toward identifying genetic defects associated with CVID.
More Related Videos
08:26The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
Published on: December 14, 2016
09:26In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Published on: October 15, 2013
