Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas
David Gajzer1, Emily Glynn1, David Wu1
1Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA.
Insights
Multiparametric flow cytometry rapidly identifies abnormal lymphoid cells for disease classification. This method is crucial for diagnosing B, T, NK, and Hodgkin lymphomas in clinical and research settings.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- Multiparametric flow cytometry (MFC) is a key diagnostic technology.
- It enables rapid assessment of cellular antigen expression.
- Immunophenotypic information is vital for disease classification.
Purpose of the Study:
- To outline a general approach for identifying abnormal lymphoid populations using MFC.
- To cover B, T, NK, and Hodgkin lymphoma cells.
- To support clinical and research applications.
Main Methods:
- Describing common antigen expression patterns of normal lymphoid cells.
- Detailing procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas.
- Presenting a strategy for diagnosing classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and T-cell/histiocyte-rich large B-cell lymphoma.
Main Results:
- Established a framework for identifying abnormal lymphoid populations via MFC.
- Provided insights into antigen expression for normal and malignant lymphocytes.
- Outlined specific diagnostic strategies for various lymphoma types.
Conclusions:
- MFC is essential for accurate lymphoid malignancy diagnosis and classification.
- Understanding normal cell antigen expression is critical for identifying aberrant populations.
- The described approach facilitates sensitive and specific lymphoma diagnosis.
Abstract:
Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow cytometry, including B, T, NK, and Hodgkin lymphoma cells suitable for the clinical and research environment. Knowledge of the common patterns of antigen expression of normal lymphoid cells is critical to permit identification of abnormal populations at disease presentation and for minimal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and T-cell/histiocyte-rich large B-cell lymphoma.
