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Published on: February 8, 2016
[Evaluation of whole blood immunoreactivity]
I A Seledtsova1, T Ya Lyubavskaya1, A A Pyshenko1
1Petrovsky National Research Center of Surgery, Moscow, Russia.
Insights
This study shows lipopolysaccharide (LPS) and Concanavalin A (Con A) stimulate different immune responses in whole blood. The findings offer insights into assessing blood immunoreactivity for clinical applications.
Area of Science:
- Immunology
- Cellular Biology
Context:
- Assessing cellular immunoreactivity is crucial for understanding immune responses.
- Whole blood assays provide a model for studying immune cell activation.
- Cytokine profiles reflect specific immune cell stimulation.
Purpose:
- To investigate the differential effects of lipopolysaccharide (LPS) and Concanavalin A (Con A) on human whole blood cytokine production.
- To evaluate the impact of heparin on immune responses stimulated by LPS and Con A.
- To explore the utility of this assay for assessing innate and adaptive blood immunoreactivity.
Summary:
- Lipopolysaccharide (LPS) preferentially stimulated the release of tumor necrosis factor-alfa (TNF-α), interleukin-6 (IL-6), IL-10, and vascular endothelial growth factor (VEGF).
- Concanavalin A (Con A) significantly enhanced the secretion of interferon-gamma (IFN-γ) and IL-2.
- Heparin addition showed a minor decrease in IL-2 and VEGF secretion, without affecting other measured cytokines.
Impact:
- This assay can differentiate immune responses to various stimuli in whole blood.
- The findings support the use of this method for objective immunologic monitoring.
- Potential applications include the preparation of blood components in surgical practice.
Abstract:
To assess cellular immunoreactivity, lipopolysaccharide (LPS), Concanavalin A (Con A), or LPS together with Con A was added to the whole blood for 18 hours. LPS preferentially stimulated release of tumor necrosis factor-alfa (TNF-α), interleukin-6 (IL-6), IL-10 and vascular endothelial growth factor (VEGF) by blood cells, whereas Con A significantly enhanced secretion of interferon-gamma (IFN-gamma) and IL-2. Addition of heparin to blood slightly decreased cellular secretion of IL-2 and VEGF, but not other cytokines. This test can be used to assess innate and adaptive blood immunoreactivity and may be useful in surgical practice for preparation of blood components (serum, plasma, leukocyte- and platelet-rich plasma) and objective immunologic monitoring.
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