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Lateral Flow Immunosensing of Salmonella Typhimurium Cells in Milk: Comparing Three Sequences of Interactions

Nadezhda A Byzova1, Irina V Safenkova1, Alexey A Gorbatov2

  • 1A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

Microorganisms
|January 8, 2025
PubMed

Insights

A new lateral flow immunoassay (LFIA) detects Salmonella Typhimurium in milk faster and more accurately. This rapid, non-laboratory method enhances food safety by enabling quicker decisions on pathogen contamination.

Area of Science:

  • Food Science
  • Microbiology
  • Immunology

Background:

  • Food safety relies on detecting pathogenic contaminants like Salmonella Typhimurium.
  • Lateral flow immunoassay (LFIA) offers a rapid, simple, and specific method for pathogen detection.

Purpose of the Study:

  • To develop and compare three LFIA formats for Salmonella Typhimurium detection in milk.
  • To optimize LFIA for efficient, non-laboratory food safety monitoring.

Main Methods:

  • Three distinct LFIA formats (A, B, C) were developed and tested for Salmonella Typhimurium.
  • Format A: common sandwich LFIA with pre-applied immunoreagents.
  • Formats B and C involved sequential incubation and lateral flow steps.

Main Results:

  • Format A demonstrated the best performance with a detection limit of 3 × 10^4 cells/mL and an assay time of 10 minutes.
  • The optimized LFIA (Format A) successfully detected Salmonella in milk samples diluted to 1.0% fat content.
  • Detection showed 70-110% recovery and 1.5-8.5% accuracy.

Conclusions:

  • The developed LFIA provides sensitive and rapid detection of Salmonella Typhimurium in milk.
  • This method accelerates decision-making in food safety control, improving overall safety.
  • LFIA is a viable technique for widespread, non-laboratory monitoring of foodborne pathogens.

Keywords:
Salmonellafood safetyimmunochromatographyorder of immunoreactants interactionstest strips

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