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[Expert consensus on flow cytometry-based assays for SARS-CoV-2-specific T cells and related operating procedure]
Insights
Standardized methods are crucial for assessing SARS-CoV-2-specific T cells, vital for understanding antiviral immunity. This consensus provides guidelines for consistent and comparable T-cell detection across laboratories.
Area of Science:
- Immunology
- Virology
- Infectious Diseases
Context:
- T-cell immune responses are critical for antiviral immunity and evaluating protective immunity.
- Current lack of standardized assays for antigen-specific T cells hinders consistent evaluation.
- Assessing SARS-CoV-2-specific T cells is necessary during and after the COVID-19 pandemic.
Purpose:
- To establish standardized detection methods and operating procedures for SARS-CoV-2-specific T cells.
- To reach a consensus on detection methods, biomarker combinations, SOPs, quality control, and data interpretation.
- To ensure consistency and comparability of results across different laboratories.
Summary:
- Experts organized by the Chinese Society of Immunology developed a consensus on detecting SARS-CoV-2-specific T cells.
- The consensus covers detection methods, biomarker selection, technical protocols, quality control, and data analysis.
- It aims to standardize the evaluation of T-cell responses to SARS-CoV-2.
Impact:
- Provides guiding significance for establishing standard detection methods and operating procedures for SARS-CoV-2-specific T cells.
- Enhances the consistency and comparability of results from various laboratories.
- Offers a reference for developing standard detection methods for antigen-specific T cells against other pathogens, like influenza.
Abstract:
T-cell immune response is an important component of antiviral immunity, it is of great significance to determine their absolute counts, relative frequencies and functionalities for evaluating protective immunity in individuals and population. However, there is a lack of guidelines or a consensus on assays for antigen-specific T cells. It is necessary to evaluate the SARS-CoV-2-specific T cells in population during and after COVID-19 epidemic. To standardize the detection method for SARS-CoV-2-specific T cells, the Chinese Society for Immunology organized experts and reached a consensus on the detection method, biomarker combination scheme, technical points of SOP, quality control, data analysis and interpretation of results, personnel training, etc. The consensus is of guiding significance to establish standard detection methods and operating procedures for SARS-CoV-2-specific T cells, which is beneficial for the consistency and comparability of results from different laboratories, and also provides reference for antigen-specific T cell standard detection methods for other pathogens (such as influenza) infection.
