Related Experiment Video
Updated: May 24, 2025
Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Immunophenotyping of B-Cell Subpopulations by Flow Cytometry
1University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, Bern, Switzerland.
Insights
This chapter details a flow cytometry protocol for immunophenotyping B-cell subsets. Accurate analysis of B-cell populations aids in diagnosing immunodeficiencies and monitoring therapies.
Area of Science:
- Immunology
- Clinical Diagnostics
Background:
- Flow cytometry is crucial for analyzing immune cells.
- B-cell subsets play key roles in immunity and disease.
Purpose of the Study:
- To present a standardized protocol for B-cell subset immunophenotyping using flow cytometry.
- To provide a resource for accurate B-cell analysis in research and clinical settings.
Main Methods:
- Detailed methodology for antibody panel selection.
- Sample preparation techniques for optimal B-cell analysis.
- Data analysis strategies for B-cell subpopulations.
Main Results:
- The protocol enables accurate quantitative and qualitative determination of B cells.
- Enables identification of specific B-cell subsets for diagnostic and research purposes.
Conclusions:
- This flow cytometry protocol is essential for understanding B-cell diversity and function.
- It supports the diagnosis of conditions like common variable immunodeficiency and monitoring B-cell depletion therapies.
Abstract:
This chapter introduces a protocol for immunophenotyping B-cell subsets using flow cytometry, a vital technique in both immunology research and clinical practice. Flow cytometry offers detailed insights into B-cell diversity and function, aiding in understanding B-cell development and guiding therapeutic decisions. This chapter delves into critical aspects such as antibody panels, sample preparation, and data analysis, all of which are essential for effectively analyzing B-cell subpopulations in diagnosing conditions like common variable immunodeficiency and monitoring therapeutic outcomes after B-cell depletion. The described protocol ensures accurate quantitative and qualitative determination of total B cells and subpopulations, providing a valuable resource for researchers and clinicians alike.
