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All-in-one detecting dairy iodine based on single drop microextraction coupled with ratiometric fluorescence filter
Ruiqi Gao1, Simin Ye2, Xiaofang Yang1
1Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
Insights
A new portable device enables sensitive detection of iodine in dairy products using rapid digestion and microextraction. This innovation aids in monitoring dietary iodine intake, crucial for preventing related diseases.
Area of Science:
- Analytical Chemistry
- Food Science
- Biomedical Engineering
Background:
- Accurate iodine detection in dairy is vital for public health, preventing deficiency and excess-related diseases.
- Developing rapid, portable, and sensitive assays for home-based dairy analysis remains a significant challenge.
Purpose of the Study:
- To develop an integrated portable device for rapid and sensitive iodine detection in dairy products.
- To address the limitations of current methods in home-based iodine content analysis.
Main Methods:
- An integrated portable device combining sample digestion, microextraction, and fluorescence signal reading was developed.
- Utilized rapid thermal-assisted digestion (500 μL sample volume) and headspace single-drop microextraction for high enrichment.
- Employed a ratiometric fluorescence filter effect system coupled with a smartphone for signal detection.
Main Results:
- Achieved a sensitive detection limit of 7 nM for iodine under optimal conditions.
- The system demonstrated minimal matrix interference, ensuring reliable measurements.
- Validated accuracy through real sample analyses and comparison with standard methods.
Conclusions:
- The developed portable device offers a practical and sensitive tool for monitoring iodine content in dairy products.
- This method is particularly valuable for assessing dietary iodine intake, especially in infant nutrition.
- Facilitates home-based analysis, improving accessibility for public health monitoring.
Abstract:
Accurately detecting iodine in dairy products is paramount to ensure adequate intake and prevent iodine excess or deficiency-related diseases. However, the development of rapid sample digestion-coupled portable sensitive assays represents a significant challenge in the home-based analysis of iodine content in dairy products. Herein, an integrated portable device was developed, which combines sample digestion, microextraction, and fluorescence signal reading. The system ensures highly sensitive detection with minimal matrix interference by utilizing a rapid thermal-assisted digestion technique with minimal sample volume (500 μL) and high-enrichment headspace single-drop microextraction. Under optimal conditions, the ratiometric fluorescence filter effect system, coupled with a smartphone, gives a detection limit of 7 nM for iodine. Moreover, the accuracy of the strategy is further validated by real sample analyses and standard method comparisons. This method provides a practical tool for monitoring dietary iodine intake, particularly infant nutrition.
