Related Experiment Video

Updated: May 5, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.0K

Quantification and clustering of immune states in hepatitis B Cirrhosis

Wei Hou1, Tengxiao Liang2, Fangliang Xing3

  • 1First Department of The Liver Disease Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China.

Immunobiology
|June 29, 2025
PubMed

Insights

Hepatitis B Cirrhosis alters lymphocyte balance, showing an inverse T/NK to B cell relationship. A mathematical model and distinct immune states aid disease staging and personalized treatment.

Area of Science:

  • Immunology
  • Virology
  • Mathematical Modeling

Background:

  • Hepatitis B Cirrhosis is a severe outcome of chronic Hepatitis B infection.
  • Understanding lymphocyte population dynamics is crucial for managing this condition.

Purpose of the Study:

  • To quantify and visualize the relationships among T cells, NK cells, and B cells in Hepatitis B Cirrhosis.
  • To develop a mathematical model for assessing immune status and optimizing treatment.

Main Methods:

  • Peripheral blood samples from 500 Hepatitis B Cirrhosis patients and 500 healthy controls.
  • Sort visualization analysis and 3D numerical fitting to model lymphocyte subset relationships.
  • Self-Organizing Feature Maps (SOFM) for unsupervised clustering of immune states.

Main Results:

  • An inverse relationship was observed: T+NK cell levels decreased as B cell levels increased.
  • SOFM identified three distinct immune state clusters.
  • A mathematical model (T% = -0.9879*B% - 1.041*NK% + 97.66) showed significant differences between patients and controls.
  • Immune states exhibited a cyclical pattern across Child-Pugh grades, correlating with disease progression.

Conclusions:

  • A quantitative model and visual representation of lymphocyte dynamics in Hepatitis B Cirrhosis were established.
  • Distinct immune states linked to disease progression aid in assessing immunological condition.
  • Integrating immunological and clinical data enables precise disease staging and personalized medicine.
Abstract

Keywords:
Hepatitis B CirrhosisImmune statesLymphocyte PopulationsUnsupervised Clustering

More Related Videos

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma08:25

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

304
A Data-Driven Approach to Quantifying Immune States in Sepsis07:42

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

319

Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.
99
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion...
28
Cirrhosis I: Introduction01:23

Cirrhosis I: Introduction

Cirrhosis is a chronic, irreversible liver disease characterized by the widespread replacement of healthy liver tissue with fibrotic scar tissue and the formation of regenerative nodules.Etiology of cirrhosisCirrhosis results from sustained liver injury that triggers progressive fibrosis and structural remodeling. The underlying causes are diverse, encompassing common and less frequent clinical conditions. Regardless of the origin, all causes lead to chronic inflammation, hepatocyte loss, and...
30
Cirrhosis II: Pathophysiology01:24

Cirrhosis II: Pathophysiology

Cirrhosis is a progressive chronic liver injury caused by prolonged inflammation, excessive fibrotic remodeling, and impaired regeneration. Over time, repeated hepatic insults disrupt the liver’s architecture and function, leading to reduced blood flow, impaired bile drainage, and diminished metabolic capacity.Pathophysiology of cirrhosisCirrhosis arises from three main responses to chronic liver damage: inflammation, immune activation, and hepatocyte death. These processes lead to...
49