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Updated: Sep 13, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

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Relationship between Urine Protein-To-Creatinine Ratio and Leukocyte Esterase in Canine Urine Samples

Chen-Yin Liao1, Tsun-Li Cheng2, Cheng-Hung Lai1,2

  • 1Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.

Veterinary Medicine and Science
|July 30, 2025
PubMed

Insights

The urine leukocyte esterase test does not impact the urine protein-to-creatinine (UP/C) ratio in dogs. This means the UP/C ratio can be reliably measured irrespective of leukocyte esterase results.

Area of Science:

  • Veterinary Medicine
  • Clinical Pathology
  • Canine Health

Background:

  • Urine dipstick tests detect leukocytes and protein, but accuracy can be affected by factors like specific gravity, pH, and infection.
  • Concerns exist about white blood cells potentially interfering with the urine protein-to-creatinine (UP/C) ratio, a crucial test for proteinuria in dogs.

Purpose of the Study:

  • To investigate whether leukocyte esterase test results influence the UP/C ratio in canine urine samples.
  • To determine if a positive leukocyte esterase test interferes with the accuracy of the UP/C ratio in dogs.

Main Methods:

  • 186 canine urine samples were analyzed.
  • Samples were categorized as leukocyte strip-positive or leukocyte strip-negative using the dipstick test.
  • The UP/C ratio was measured using the Catalyst One biochemical analyzer.

Main Results:

  • No significant correlation was found between leukocyte strip status and the UP/C ratio (correlation coefficient = 0.014, p = 0.849).
  • Analysis using International Renal Interest Society (IRIS) grading criteria also showed no significant association between leukocyte strip status and abnormal UP/C ratios.
  • Further categorization by IRIS criteria confirmed no link between leukocyte strip status and UP/C classification (correlation coefficient = 0.032, p = 0.661).

Conclusions:

  • A positive leukocyte esterase test does not affect the UP/C ratio in canine urine.
  • The UP/C ratio test is reliable for assessing proteinuria in dogs, regardless of leukocyte esterase test outcomes.
Abstract

Keywords:
UP/C ratiocanineleukocyte esteraseproteinuriaurinalysisurine dipstick strip

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