Increase CD24+CD27+ B cells within pleural effusions derived from lung adenocarcinoma presents enhanced potential for
Yueming Liang1, Danqi Sun2, Qizhi Xu2
1Department of Geriatric Respiratory Medicine, Guangdong Provincial Geriatrics Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical, Sciences, Southern Medical University, Guangzhou, Guangdong, China; Department of Respiratory and Critical Care Medicine, The First People's Hospital of Foshan, Foshan, Guangdong, China.
Insights
Regulatory B cells (Bregs) are elevated in lung adenocarcinoma pleural effusions (PE). These CD24+CD27+ B cells show altered markers and IL-10 upregulation, suggesting potential diagnostic and therapeutic roles in lung cancer.
Area of Science:
- Immunology
- Oncology
Background:
- The role of B cells, especially regulatory B cells (Bregs), in the tumor microenvironment is gaining attention.
- Immunological characteristics of B cells in malignant pleural effusions (PE) are not well understood.
Purpose of the Study:
- To investigate the immunological phenotypes and functions of B cell subsets in malignant pleural effusions (PE) from lung adenocarcinoma (LUAD).
- To assess the potential of CD24+CD27+ B cells as diagnostic markers and therapeutic targets in LUAD.
Main Methods:
- Flow cytometry analysis of 143 pleural effusion and peripheral blood samples.
- Assessment of B and T cell proportions, surface markers (CD38, CD5, CD71, PD-1, PD-L1, CD39), and cytokines (IFN-γ, IL-10).
- Comparison of CD24+CD27+ B cell populations in LUAD-PE versus tuberculosis (TB)-PE and peripheral blood.
Main Results:
- B cell populations were significantly higher in LUAD-PE compared to TB-PE.
- CD24+CD27+ B cells were markedly elevated in LUAD-PE, with reduced CD38, CD5, CD71, PD-1, PD-L1, and increased IL-10 and CD39 expression.
- Significant relationships were observed between CD24+CD27+ B cells and clinical measurements.
Conclusions:
- B cell subsets in LUAD-PE exhibit heterogeneous phenotypes and functions.
- CD24+CD27+ B cells represent potential diagnostic markers in LUAD.
- Further research into the immunoregulatory functions of these B cells may reveal novel immunotherapeutic strategies for lung cancer.
Background:
The increasing interest in the roles of B cells, particularly regulatory B cells, within the tumor microenvironment has become prominent, though their immunological characteristics in malignant pleural effusions (PE) remain poorly elucidated.
Methods:
Flow cytometry was performed in 143 pleural effusion and peripheral blood samples from patients in order to analyze the proportions ofPB-derived B and T cells, and to assess surface markers and cytokines, such as IFN-γ and IL-10. Moreover, we compared clinical role of CD24+CD27+B cell populations.
Results:
B cell populations were significantly higher in PE from lung adenocarcinoma (LUAD)than tuberculosis (TB). Compared to PB from patients, the proportion of CD24+CD27+ B cells was markedly elevated in LUAD-PE and exhibited reduced levels of CD38, CD5, CD71,PD-1, and PD-L1, as well as an upregulation of IL-10 and CD39. PD-1 and PD-L1 werelargely found to be upregulated within the CD27+CD38+ B cell subset despite declining proportions overall. Re-interpretations illustrated significant relationshipsbetween CD24+CD27+ B cells and clinical measurements.
Conclusions:
This study highlights the heterogenicphenotypes and functions of various B cell subsets in LUAD-PE, with specific attention to CD24+CD27+ B cells as potential diagnostic markers and the need for further studies investigating their immunoregulatory functions to unveil new immunotherapeutic strategies in lung cancer.
