Related Experiment Video
Updated: May 5, 2026
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Published on: April 19, 2017
A practical approach to panel design, validation, and interpretation for the evaluation of T-cell neoplasms by flow
Jean Oak1, Felipe Magalhaes Furtado2,3, Katherine A Devitt4
1Department of Pathology, Stanford University, Stanford, California, USA.
Insights
This guide offers a practical framework for diagnosing T-cell neoplasms using immunophenotyping. It details key antigens like TRBC1 and TRBC2, and analytical methods to improve accuracy in T-cell leukemia and lymphoma assessment.
Area of Science:
- Hematopathology
- Immunophenotyping
- T-cell Neoplasms
Background:
- T-cell neoplasms present diagnostic challenges due to immunophenotypic heterogeneity.
- Accurate diagnosis is crucial for effective treatment and patient outcomes.
Purpose of the Study:
- To provide a practical framework for T-cell neoplasm immunophenotyping.
- To standardize laboratory assessment of these challenging hematologic malignancies.
Main Methods:
- Outlines utility of key antigens (TRBC1, TRBC2) for screening and classification.
- Discusses analytical strategies like "difference from normal" and template-based gating.
- Incorporates validation considerations aligned with CLSI H62 guidelines.
Main Results:
- Provides a framework for designing, validating, and interpreting immunophenotyping studies.
- Highlights specific antigens and methods for improved T-cell neoplasm assessment.
Conclusions:
- Standardizing immunophenotyping workflows enhances T-cell neoplasm assessment.
- This best practice guide aims to improve diagnostic accuracy across clinical settings.
Abstract:
The diagnosis of T-cell neoplasms remains one of the most challenging areas in hematopathology due to the immunophenotypic heterogeneity and subtle aberrancies often present in these entities. This "Best Practice" manuscript provides a practical framework for laboratories to design, validate, and interpret immunophenotyping studies of immature and mature T-cell neoplasms. We outline the utility of key antigens in the screening and classification of T-cell lymphomas/leukemia including TRBC1 and TRBC2. Analytical strategies using the "difference from normal" method and template-based gating are discussed, along with validation considerations aligned with CLSI H62 guidelines. By integrating these principles into laboratory workflows, this manuscript aims to standardize and improve the assessment of T-cell neoplasms across diverse clinical settings.
