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Updated: Jan 13, 2026
Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
A Simple and Efficient Method for Analyzing Murine Gingival Immune Cells Using Flow Cytometry: An Animal Study
Wenting Jiang1,2, Xuekang Wang3, Yiping Wei1
1Department of Periodontology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Insights
Perfusion is not necessary for flow cytometry analysis of immune cells in mouse gingival tissue, simplifying sample preparation for periodontitis research.
Area of Science:
- Immunology
- Periodontology
- Cell Biology
Background:
- Understanding the immune cell network is crucial for periodontitis pathogenesis.
- Flow cytometry is essential for quantifying immune cells during inflammation.
- Perfusion is typically required before tissue harvesting for flow cytometry, but its necessity for murine gingiva was unstudied.
Purpose of the Study:
- To investigate whether perfusion is necessary for accurate flow cytometric analysis of immune cell composition in murine gingival tissues.
- To establish an efficient protocol for processing murine gingival tissue for immunological studies.
Main Methods:
- Experimental periodontitis was induced in mice using ligation.
- Mice were divided into perfused and non-perfused groups, with and without periodontitis.
- Immune cell composition in palatal gingival tissues was analyzed using flow cytometry.
Main Results:
- Experimental periodontitis led to alveolar bone resorption and inflammatory cell infiltration.
- Perfusion effectively removed blood from murine periodontal vessels.
- No significant differences in immune cell proportions were observed between perfused and non-perfused gingival tissues.
Conclusions:
- This study is the first to demonstrate that perfusion is not required for flow cytometry analysis of immune cells in mouse gingiva.
- The findings simplify sample processing for immunological studies of periodontal disease.
- This facilitates research into the immune cell network's role in periodontitis.
Background:
Elucidating the immune cell network is of great significance for understanding the pathogenesis of periodontitis. Flow cytometry is the preferred tool for quantifying the composition of immune cells in the pathological process of inflammation. Perfusion prior to tissue harvesting is necessary for flow cytometric analysis of various tissues. However, whether perfusion is necessary for flow cytometric analysis of murine gingival tissues has never been reported.
Method:
Forty-eight mice were randomly divided into four groups: control + non-perfused group, control + perfused group, periodontitis + non-perfused group, and periodontitis + perfused group. Ligation-induced experimental periodontitis was established in periodontitis mice. The circulating blood was perfused out in the perfused groups before harvesting tissue samples. Flow cytometry was used to analyze the composition of immune cells in murine palatal gingival tissues.
Results:
Experimental periodontitis induced alveolar bone resorption and inflammatory cell infiltration. Perfusion can successfully remove blood from the vessels of murine periodontal tissues. Flow cytometric analysis showed that there was no significant difference in the proportion of various immune cells in murine gingival tissues between the perfusion groups and the non-perfusion groups.
Conclusion:
The present study was the first to use flow cytometry to compare perfused mouse gingiva with non-perfused mouse gingiva and found no significant difference in immune cell composition between the two groups. In addition, this study provides a simple and efficient protocol for the collection and processing of murine gingival tissue, which could provide facilities for understanding the role and mechanism of the immune cell network in periodontal diseases.
