Related Experiment Video

Updated: Aug 8, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

Sorting and retrieval between the endoplasmic reticulum and Golgi apparatus

H R Pelham1

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Current Opinion in Cell Biology
|August 1, 1995
PubMed

Insights

Retrieval of endoplasmic reticulum membrane proteins relies on COPI coat proteins. Research also explored the intermediate compartment and protein concentration mechanisms in transport.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • Endoplasmic reticulum (ER) membrane proteins require specific mechanisms for retrieval.
  • The nature of the 'intermediate compartment' in protein transport remains debated.
  • Understanding protein concentration during transport is crucial for cellular function.

Purpose of the Study:

  • To demonstrate the involvement of COPI coat proteins in the retrieval of ER membrane proteins.
  • To contribute to the understanding of the intermediate compartment's role in protein transport.
  • To elucidate the mechanism of protein concentration during ER-to-Golgi transport.

Main Methods:

  • Investigated the role of specific protein motifs (di-lysine) in protein retrieval.
  • Utilized techniques to study the 'intermediate compartment' and protein transport dynamics.
  • Analyzed protein concentration mechanisms at the ER-Golgi interface.

Main Results:

  • Demonstrated that COPI coat proteins are essential for the retrieval of ER membrane proteins with the di-lysine motif.
  • Provided new data on the characteristics of the intermediate compartment.
  • Offered insights into how transported proteins are concentrated.

Conclusions:

  • COPI coat proteins play a direct role in the retrograde transport of specific ER membrane proteins.
  • The findings advance the discussion on the functional identity of the intermediate compartment.
  • Elucidated aspects of protein sorting and concentration in the secretory pathway.

More Related Videos

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

Related Concept Videos

Endoplasmic Reticulum01:39

Endoplasmic Reticulum

Endoplasmic ReticulumThe endoplasmic reticulum (ER) is an extensive network of membranous sacs and tubules in eukaryotic cells, continuous with the outer membrane of the nucleus. This structural continuity integrates nuclear and cytoplasmic processes and facilitates efficient intracellular transport. This allows mRNA to move directly from the nucleus to ribosomes for efficient protein synthesis. As a result, the ER serves as a central site for the synthesis, processing, and distribution of...
Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Transport Across the Golgi01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...