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Not only Th2 cells but also Th1 and Th0 cells express CD30 after activation

A Bengtsson1, C Johansson, M T Linder

  • 1Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden.

Journal of Leukocyte Biology
|December 1, 1995
PubMed

Insights

CD30 molecule expression was studied in T helper cell subtypes. Activated Th2 cells showed sustained CD30 expression, unlike Th1 and Th0 cells, suggesting a distinct characteristic.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The CD30 molecule is expressed on a subset of T and B lymphocytes.
  • T helper (Th) cells are crucial immune regulators with distinct subtypes (Th1, Th0, Th2).

Purpose of the Study:

  • To determine if CD30 expression defines a specific subtype of T helper cells.
  • To analyze CD30 expression kinetics in different T helper cell clones.

Main Methods:

  • Pityrosporum orbiculare-specific CD4+ T cell clones were generated and classified as Th1, Th0, or Th2 based on cytokine mRNA profiles via RT-PCR.
  • CD30 expression was assessed using flow cytometry, immunocytochemistry, and RT-PCR following anti-CD3 (OKT3) stimulation.

Main Results:

  • OKT3 activation induced high CD30 expression in Th1, Th0, and Th2 clones within 1-3 days.
  • Th2 clones maintained high CD30 expression beyond day 3, while Th1 and Th0 expression declined.
  • CD30 is expressed on activated CD4+ T cells across all three subtypes.

Conclusions:

  • CD30 is not exclusive to a single T helper subtype but is broadly expressed upon activation.
  • Sustained CD30 expression is a characteristic feature distinguishing Th2 cells from Th1 and Th0 cells.

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