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Immunohistochemical detection of CD1A antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal
L Krenács1, L Tiszalvicz, T Krenács
1Department of Pathology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Insights
A new CD1a monoclonal antibody (MAb) 010 shows reliable immunoreactivity in formalin-fixed tissues. This antibody identifies various dendritic cells and thymocytes, even in paraffin-embedded samples.
Area of Science:
- Immunohistochemistry
- Cellular immunology
- Pathology
Background:
- CD1a antigen is crucial for identifying specific immune cells.
- Previous CD1a monoclonal antibodies (MAbs) often require fresh or frozen tissues.
- Formalin fixation can alter antigenicity, complicating immunohistochemical analysis.
Purpose of the Study:
- To evaluate the immunoreactivity of a novel CD1a MAb, designated 010, in formalin-fixed, paraffin-embedded (FFPE) tissues.
- To determine if MAb 010 can reliably detect CD1a-expressing cells in routinely processed tissue samples.
- To characterize the cellular distribution of CD1a antigen in various reactive and neoplastic lymphoid conditions using MAb 010.
Main Methods:
- Streptavidin-biotin-peroxidase method was employed for immunostaining.
- Analysis was performed on 47 cases of formalin-fixed and paraffin-embedded tissue samples.
- Tissues included reactive lymphoid proliferations and various lymphomas and thymomas.
Main Results:
- MAb 010 demonstrated consistent immunostaining in FFPE sections.
- Interdigitating dendritic cells, dermal dendritic cells, veiled cells, and Langerhans' cells were positive.
- Cortical thymocytes and their neoplastic counterparts also showed positive staining with MAb 010.
- Results were comparable to those obtained with other CD1a MAbs on fresh or frozen specimens.
Conclusions:
- MAb 010 exhibits robust immunoreactivity in FFPE tissues, indicating its utility in routine diagnostics.
- The antibody recognizes a fixation-resistant epitope on the CD1a antigen.
- MAb 010 provides a valuable tool for identifying CD1a-expressing cells in a wide range of pathological conditions.
Abstract:
The immunoreactivity of a CD1a monoclonal antibody (MAb), denoted 010, was investigated by means of the streptavidin-biotin-peroxidase method in formalin-fixed and paraffin-embedded tissues from 47 cases. The samples comprised reactive lymphoid proliferations of skin, tonsil, and lymph node including dermatopathic lymphadenopathy and Langerhans' cell histiocytosis, Hodgkin's and non-Hodgkin's lymphomas, and thymomas. Interdigitating and dermal dendritic cells, veiled cells, Langerhans' cells, and also cortical thymocytes and their neoplastic counterparts displayed immunostaining with MAb 010 in paraffin sections. These results are identical to previous ones reported for other CD1a MAbs in fresh or frozen specimens. The findings suggest that the binding site of 010 is a fixation-resistant epitope of CD1a antigen which has not been previously identified.
