Related Experiment Video

Updated: Aug 11, 2026

Generation of Human CD40-activated B cells
13:27

Generation of Human CD40-activated B cells

Published on: October 17, 2009

Role of CD40 antigen and interleukin-2 in T cell-dependent human B lymphocyte growth

D Blanchard1, C Gaillard, P Hermann

  • 1Schering-Plough, Laboratory for Immunological Research, Dardilly, France.

European Journal of Immunology
|February 1, 1994
PubMed

Insights

CD40 ligand-CD40 interaction is crucial for T cell-dependent B cell responses, particularly those mediated by IL-2. However, some B cell activation, especially sIgD+ cells, may not require this specific pathway.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T cell-dependent B cell responses are vital for adaptive immunity.
  • The role of CD40 ligand (CD40L)-CD40 interaction in these responses requires further elucidation.

Purpose of the Study:

  • To investigate the specific role of CD40L-CD40 interaction in T cell-mediated B cell activation and immunoglobulin (Ig) secretion.
  • To determine the influence of B cell subsets, like sIgD+ cells, in this interaction.

Main Methods:

  • Purified B lymphocytes were co-cultured with irradiated, activated CD4+ T cells.
  • The effect of anti-CD40 monoclonal antibody (mAb) 89 on B cell proliferation and Ig secretion was assessed.
  • Differential responses of B cell subsets (sIgD+ vs. sIgD-) were analyzed.

Main Results:

  • Anti-CD40 mAb 89 significantly inhibited proliferation and Ig secretion in tonsil B cells.
  • Proliferation of surface (s)IgD+ B cells was less sensitive to anti-CD40 inhibition compared to sIgD- B cells.
  • T cell-activated B cells showed preferential response to IL-2, contrasting with the IL-4 and IL-10 dependence in the CD40 system.

Conclusions:

  • CD40L-CD40 interaction is important for IL-2 mediated T cell-dependent B cell responses.
  • A subset of sIgD+ B cells might be activated independently of the CD40L-CD40 pathway.

More Related Videos

Murine Model of CD40-activation of B cells12:24

Murine Model of CD40-activation of B cells

Published on: March 6, 2010

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation07:20

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Published on: January 15, 2011

Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...