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Updated: Aug 12, 2026
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Published on: February 16, 2017
[Immunophenotyping in chronic B-cell lymphatic leukemia]
Insights
Immunophenotyping identified characteristic surface molecules in B-cell chronic lymphocytic leukemia (B-CLL) patients. Reduced T cells and NK cells were linked to immunodeficiency in B-CLL.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- B-cell chronic lymphocytic leukemia (B-CLL) is a common hematological malignancy.
- Immunophenotyping is crucial for diagnosing and characterizing B-CLL.
- Patients with B-CLL often exhibit compromised immune function.
Purpose of the Study:
- To analyze the immunophenotypic profile of B-CLL cells.
- To investigate the relationship between B-CLL immunophenotype and immune status.
Main Methods:
- Immunophenotyping analysis was performed on peripheral blood cells.
- Flow cytometry was used to identify cell surface markers.
- Data from 67 B-CLL patients were analyzed.
Main Results:
- B-CLL cells typically express HLA DR, pan B-cell markers, and CD5.
- Aberrant expression, including the absence of some pan B-cell markers, was observed.
- A significant reduction in T lymphocytes, their subsets, and NK cells was noted in B-CLL patients.
Conclusions:
- Immunophenotyping confirms B-CLL diagnosis and reveals aberrant cell surface markers.
- Reduced T and NK cell populations in B-CLL patients correlate with increased susceptibility to infections due to immunodeficiency.
Abstract:
The authors present the results of immunophenotyping analysis of cells from 67 patients with the diagnosis of B-CLL from group of 393 adults examined by immunophenotyping because of a haematological malignity. Characteristic for B-CLL is the finding of surface molecules HLA DR, B lymphocytic superficial molecules and CD5. Aberrant membrane equipment of B-CLL cells is suggested by the rare absence of some pan B lymphocytic sign. Marked reduction of T lymphocyte ratios, their sub-populations and NK cells is associated with immunodeficiency manifestations which are common in patients with B-CLL.
