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Generation of Human CD40-activated B cells
13:27

Generation of Human CD40-activated B cells

Published on: October 16, 2009

Anti-immunoglobulin and anti-CD40 stimulation induces CD25 expression by resting human tonsillar B lymphocytes

E L Burlinson1, H M Pringle, B W Ozanne

  • 1Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, UK.

Immunology Letters
|February 1, 1995
PubMed

Insights

Interleukin-4 (IL-4) is the sole cytokine that induces CD25 expression on quiescent human B cells. Cross-linking B-cell receptors or CD40 also promotes CD25 expression, with anti-Ig being more potent than anti-CD40.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD25 is the 55-kDa inducible component of the IL-2 receptor complex.
  • CD25 is expressed on T and B lymphocytes.

Purpose of the Study:

  • To investigate the effect of B-cell surface molecule ligation on CD25 expression.
  • To identify cytokines and surface molecule stimulation that induce CD25 expression in human B cells.

Main Methods:

  • Culturing high-density quiescent tonsillar B cells.
  • Stimulating B cells with cytokines (IL-4, IL-10, IL-13) and antibodies against B-cell surface molecules (antigen receptors, CD40).
  • Assessing CD25 expression using flow cytometry.

Main Results:

  • Interleukin-4 (IL-4) was the only tested cytokine to induce CD25 expression in quiescent human B cells.
  • Antibody-mediated cross-linking of antigen receptors (anti-Ig) or CD40 induced CD25 expression in a dose-dependent manner.
  • Anti-Ig stimulation resulted in higher CD25 expression (≥80% of cells) compared to anti-CD40 (approx. 25% of cells).
  • Combinations of stimuli showed additive but not synergistic effects on CD25 expression.

Conclusions:

  • IL-4 is a key cytokine for inducing CD25 expression in quiescent human B cells.
  • B-cell activation via antigen receptors or CD40 can also upregulate CD25 expression.
  • Distinct signaling pathways may be involved in CD25 induction by different stimuli.

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