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Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain

J Xie1, R Li, P Kotovuori

  • 1Department of Biosciences, University of Helsinki, Finland.

Journal of Immunology (Baltimore, Md. : 1950)
|October 1, 1995
PubMed

Insights

Intercellular adhesion molecule-2 (ICAM-2) protein interacts with leukocyte function-associated antigen-1 (LFA-1) and Mac-1 (CD11/CD18). This study shows ICAM-2 binds to CD11b/CD18 via the CD11b A domain.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Leukocyte adhesion is crucial for immune responses.
  • Beta 2-integrins (CD11/CD18) mediate leukocyte adhesion by binding intercellular adhesion molecules (ICAMs).
  • ICAM-1 binds CD11a/CD18 and CD11b/CD18; ICAM-2 binds CD11a/CD18.

Purpose of the Study:

  • To investigate the interaction between ICAM-2 and CD11b/CD18.
  • To identify the specific binding site of ICAM-2 on CD11b/CD18.

Main Methods:

  • Protein-protein interaction assays.
  • Analysis of binding domains using purified proteins and peptides.

Main Results:

  • The ICAM-2 protein directly interacts with CD11b/CD18.
  • Binding of ICAM-2 to CD11b/CD18 is mediated by the A domain of CD11b.
  • A previously identified CD11a/CD18-binding peptide from ICAM-2 also binds CD11b/CD18.

Conclusions:

  • ICAM-2 is a ligand for both CD11a/CD18 and CD11b/CD18.
  • The CD11b A domain is critical for ICAM-2 binding.
  • These findings expand the understanding of leukocyte adhesion molecule interactions.

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