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Generation of Human CD40-activated B cells
13:27

Generation of Human CD40-activated B cells

Published on: October 16, 2009

Regulation of human B cell function by recombinant CD40 ligand and other TNF-related ligands

M D Jumper1, Y Nishioka, L S Davis

  • 1University of Texas Southwestern Medical Center at Dallas, Simmons Arthritis Research Center 75235, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1995
PubMed

Insights

CD40 ligand (CD40L) powerfully stimulates B cell proliferation and gene transcription. However, CD30 ligand (CD30L) inhibits this transcription, showing TNF family members have distinct impacts on B cell function.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The Tumor Necrosis Factor (TNF) superfamily plays critical roles in immune regulation.
  • Understanding the specific functions of TNF superfamily ligands in B cell responses is crucial for immunology research.

Purpose of the Study:

  • To investigate the stimulatory potential of CD40 ligand (CD40L) and related molecules (CD27L, CD30L, TNF-alpha) on human B cell responses.
  • To elucidate the distinct roles of these ligands in B cell proliferation and gene transcription.

Main Methods:

  • Expression of CD40L, CD27L, CD30L, and TNF-alpha in the baculovirus system.
  • Co-culture of Sf9 insect cells expressing these ligands with normal human B cells and B cell lines.
  • Assessment of B cell aggregation, proliferation, and germ-line transcription.

Main Results:

  • CD40L significantly promoted B cell aggregation, proliferation, and germ-line transcription without cytokine costimulation.
  • CD27L, CD30L, and TNF-alpha did not affect B cell proliferation.
  • CD30L inhibited germ-line transcription of the immunoglobulin epsilon gene in CD30-expressing B cells, while CD27L and TNF-alpha had no effect on germ-line transcripts.

Conclusions:

  • Different TNF family members exert specific and sometimes opposing effects on human B cell function.
  • CD40L is a potent stimulator of B cell responses, whereas CD30L can inhibit key transcriptional events.

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