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Updated: Aug 11, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Interferons upregulate the expression of laminin and its receptor LBP-32 in cultured cells

P N Raghunath1, G S Sidhu, H C Coon

  • 1Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.

Journal of Biological Regulators and Homeostatic Agents
|January 1, 1993
PubMed

Insights

Interferons (IFNs) boost the production of laminin (LMN) and laminin binding protein (LPB-32) in human cells. This enhanced synthesis may play a role in wound healing and blood vessel formation.

Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • Laminin (LMN) is a crucial extracellular matrix protein involved in cell adhesion and tissue development.
  • Laminin binding protein (LPB-32) interacts with LMN, influencing cellular processes.
  • Interferons (IFNs) are cytokines with diverse roles in immunity and cell regulation.

Purpose of the Study:

  • To investigate the impact of interferon-alpha (IFN-α) and interferon-gamma (IFN-γ) on LMN and LPB-32 expression.
  • To analyze the regulation of LMN and LPB-32 at both protein and mRNA levels.
  • To explore the potential roles of IFN-induced LMN and LPB-32 in physiological processes like wound healing and angiogenesis.

Main Methods:

  • Cell culture of human umbilical vein endothelial cells (HUVEC) and human foreskin fibroblast (FS-4) cells.
  • Immunofluorescence staining to assess LMN and LPB-32 protein levels.
  • Immunoprecipitation to quantify specific LMN B1 and B2 chain proteins in intracellular and extracellular fractions.
  • In situ hybridization and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) to analyze LMN B1 and B2 chain mRNA expression.

Main Results:

  • IFNs significantly increased immunofluorescent staining for both LMN and LPB-32 in HUVEC and FS-4 cells.
  • IFN-α enhanced extracellular LMN B1 and B2 chains in HUVEC, while IFN-γ decreased them but increased intracellular B chains.
  • Both IFNs upregulated LMN B1 chain mRNA in HUVEC, and IFN-γ upregulated B1 and B2 chain mRNA in FS-4 cells.

Conclusions:

  • Interferons alpha and gamma modulate the expression and synthesis of laminin and its binding protein.
  • IFN-induced changes in LMN and LPB-32 may contribute to enhanced wound healing and angiogenesis.
  • These findings highlight a novel role for interferons in regulating extracellular matrix components relevant to tissue repair.

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