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[In situ expression of intercellular adhesion molecule-1 in human glomerulonephritis]
Insights
Intercellular adhesion molecule-1 (ICAM-1) is elevated in diseased kidneys, correlating with glomerular inflammation and interstitial cell infiltration. This study highlights ICAM-1
Area of Science:
- Nephrology
- Immunology
- Cell Biology
Context:
- Kidney diseases involve complex cellular and molecular changes.
- Intercellular adhesion molecule-1 (ICAM-1) plays a role in immune cell trafficking and inflammation.
- Understanding ICAM-1's role in renal pathology is crucial for disease management.
Purpose:
- To investigate the presence and role of intercellular adhesion molecule-1 (ICAM-1) in normal and diseased kidneys.
- To correlate ICAM-1 expression levels with the degree of glomerular proliferation, sclerosis, and interstitial infiltration.
- To validate immunocytochemical findings using in situ hybridization.
Summary:
- Immunocytochemistry and in situ hybridization were used to analyze ICAM-1 in 64 renal biopsy specimens.
- Increased glomerular ICAM-1 expression was significantly higher in lupus nephritis compared to minimally changed disease.
- Glomerular ICAM-1 levels correlated with cellular proliferation and infiltration, while tubular ICAM-1 correlated with interstitial infiltration.
Impact:
- Glomerular ICAM-1 expression is a potential biomarker for the inflammatory degree in glomeruli.
- Tubular ICAM-1 expression is associated with interstitial cell infiltration in kidney disease.
- Findings contribute to understanding the pathogenesis of renal inflammation and potential therapeutic targets.
Abstract:
We conducted immunocytochemical analysis with in situ hybridization technique to investigate the presence and role of intercellular adhesion molecule-1 (ICAM-1) in normal and diseased kidneys. A total of 64 renal biopsy specimens were classified in three groups according to the degree of cellular proliferation in glomerulus. Group A presented no or mild proliferation (n = 38). Group B showed definite proliferation (n = 21). Group C had glomerular sclerosis (n = 5). The ratio of cases exhibiting increased glomerular ICAM-1 expression in lupus nephritis (9/10) was significantly different from that in minimally changed disease (1/5) (P = 0.017). Glomerular ICAM-1 expression level correlated well with the cellular proliferation and infiltration in glomerulus (B&A: P < 0.001; C&B: P < 0.05). The percentage of cases with tubular ICAM-1 positivity in group B (81.0%) and group C (100%) was greater than that in group A (52.6%), which was also correlated with that of interstitial cell infiltration. In situ hybridization of 9 renal biopsies with digoxigenin labelled oligo probe proved immunocytochemical findings. We conclude that glomerular ICAM-1 expression level is correlated with inflammatory degree of glomerulus and that tubular ICAM-1 expression is associated with interstitial cell infiltration.