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CD82, tetra-span-transmembrane protein, is a regulated transducing molecule on U937 monocytic cell line

S Lebel-Binay1, C Lagaudrière, D Fradelizi

  • 1Laboratoire d'Immunomodulation et Autoimmunité, Institut National de la Santé et de la Recherche Médicale, Hôpital Cochin, Paris, France.

Journal of Leukocyte Biology
|June 1, 1995
PubMed

Insights

The study identifies CD82 as a mononuclear cell marker that, when cross-linked with Fc receptors, enhances calcium signaling and cell activation. This suggests CD82 plays a role in monocyte/macrophage activation via Fc receptor pathways.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD82 (IA4) is a mononuclear cell surface protein belonging to the tetra spans transmembrane (TST) family.
  • CD82 functions as an activation/differentiation marker for mononuclear cells.
  • The TST protein family includes CD9, CD37, CD53, CD63, and CD81.

Purpose of the Study:

  • To characterize CD82 as a novel activation marker for mononuclear cells.
  • To investigate the signaling pathways induced by CD82 cross-linking.
  • To explore the role of CD82 in Fc receptor-mediated cellular responses.

Main Methods:

  • Monoclonal antibody (mAb) cross-linking of CD82 on U937 cells.
  • Measurement of intracellular free calcium and tyrosine phosphorylation.
  • Cytokine treatment to assess CD82 surface expression changes.
  • Comparison of CD82 and Fc receptor (FcR) cross-linking effects.

Main Results:

  • Cross-linking CD82 mAbs induced intracellular calcium increase and protein tyrosine phosphorylation in U937 cells.
  • Calcium signaling involved phospholipase C (PLC) and extracellular calcium entry, dependent on both CD82 and FcR engagement.
  • Dual cross-linking of CD82 and FcRs resulted in sustained calcium increase, unlike FcR cross-linking alone.
  • Cytokines like IL-1β, IL-4, IL-6, IL-13, IFN-γ, and TNF-α up-regulated CD82 surface expression.

Conclusions:

  • CD82, upon cross-linking with FcRs, can form a multimolecular complex to trigger signal transduction.
  • CD82 may enhance FcR-mediated activation in monocyte/macrophage lineage cells.
  • CD82 is a key player in immune cell activation and differentiation pathways.

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