Related Experiment Video

Updated: Jun 24, 2026

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
09:49

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Published on: March 17, 2013

Peyer's patch CD8+ memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin

A S Lagoo1, J H Eldridge, S Lagoo-Deenadaylan

  • 1Department of Medicine (Division of Rheumatology and Clinical Immunology), University of Mississippi Medical Center, Jackson.

European Journal of Immunology
|December 1, 1994
PubMed

Insights

Murine Peyer's patches harbor activated CD8+ T cells that secrete T helper type 2 cytokines, including interleukin-4 (IL-4), IL-5, and IL-10. These cells provide functional help for B cells, enhancing immunoglobulin (Ig) production at mucosal sites.

Area of Science:

  • Immunology
  • Mucosal immunology

Background:

  • Peyer's patches (PP) exhibit high spontaneous cytokine gene expression, indicative of active germinal centers and constant antigen exposure.
  • CD8+ T cells in PP differ phenotypically from those in spleen and mesenteric lymph nodes (MLN).

Purpose of the Study:

  • To investigate cytokine gene expression in murine Peyer's patches (PP).
  • To characterize CD8+ T cells in PP and compare them to spleen and MLN CD8+ T cells.
  • To determine the functional capacity of PP CD8+ T cells in supporting B cell responses.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) for cytokine gene expression analysis.
  • Flow cytometry for purification and phenotyping of CD8+ T cells (CD45RB expression).
  • In vitro stimulation assays with anti-CD3 antibody and co-culture with B cells.

Main Results:

  • PP showed higher spontaneous cytokine transcript levels than spleen and MLN.
  • PP CD8+ T cells expressed transcripts for IL-4, IL-5, and IL-10, and predominantly displayed an activated/memory phenotype (CD45RBlo).
  • PP and MLN CD8+ T cells secreted IL-5 and IL-10 upon stimulation and enhanced IgG and IgA secretion from B cells.

Conclusions:

  • CD8+ T cells at mucosal sites, specifically in PP, exhibit a T helper type 2 cytokine profile.
  • These mucosal CD8+ T cells possess functional capacity to provide help to B cells, supporting immunoglobulin production in these tissues.

More Related Videos

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches08:25

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Published on: November 21, 2018

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...