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Published on: January 15, 2011
Immunosuppression by lymphokine-activated murine killer cell line with B-lymphoblast-lytic activity in vitro
M Ikemoto1, H Suzuki, E Sugiyama
1First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Insights
Murine lymphokine-activated killer (LAK) cells, specifically the BC-1.10 clone, demonstrate immunosuppressive effects by eliminating B lymphoblasts. This finding suggests a potential role for LAK cells in regulating immune responses through immunosuppression.
Area of Science:
- Immunology
- Cell Biology
Background:
- Lymphokine-activated killer (LAK) cells are crucial in immune responses.
- The specific function of LAK cells in immunosuppression requires further investigation.
Purpose of the Study:
- To investigate the in vitro immunosuppressive effect of a murine LAK cell line (BC-1.10) with B-lymphoblast-lytic activity.
- To elucidate the mechanism underlying the observed immunosuppression.
Main Methods:
- Utilized a cloned murine LAK cell line (BC-1.10) with defined phenotype (Thy 1.2+, LFA-1+, TCR-alpha beta-, TCR-gamma delta-, Fc gamma RII-, CD2-, CD3 epsilon-, CD4-, CD8-, zeta chain mRNA+).
- Assessed the effect of BC-1.10 cells on lipopolysaccharide (LPS)-induced immunoglobulin (Ig) synthesis by B lymphoblasts.
- Employed phase-contrast microscopy to observe B lymphoblast viability.
- Investigated the role of LFA-1 in the suppressive effect using anti-LFA-1 monoclonal antibody (mAb) pretreatment.
Main Results:
- BC-1.10 cells significantly suppressed LPS-induced Ig synthesis by B lymphoblasts.
- Phase-contrast microscopy revealed the disappearance of B lymphoblasts within 24 hours of BC-1.10 cell addition.
- Pretreatment of BC-1.10 cells with anti-LFA-1 mAb reduced their suppressive effect, indicating LFA-1's role in cytotoxicity.
Conclusions:
- The immunosuppressive effect of BC-1.10 cells is mediated by the elimination of B lymphoblasts.
- These findings suggest that LAK cells may play a physiological role in immunosuppression as part of the immune response.
Abstract:
The in vitro immunosuppressive effect caused by a murine lymphokine-activated killer cell line with B-lymphoblast-lytic activity was studied. The cloned cells (named BC-1.10, phenotype Thy 1.2+, LFA-1+, TCR-alpha beta-, TCR-gamma delta-, Fc gamma RII-, CD2-, CD3 epsilon-, CD4-, CD8- and express mRNA of zeta chain) suppressed LPS-induced Ig synthesis by B lymphoblasts previously stimulated with LPS. Phase-contrast microscopy indicated disappearance of B lymphoblasts at 24 h after the addition of BC-1.10 cells. This suppressive effect was reduced when BC-1.10 cells were pretreated with anti-LFA-1 mAb, which inhibits cytotoxicity of this clone. These data suggest that the immunosuppressive effect of BC-1.10 is due to an elimination of B lymphoblasts, and that one of the physiological functions of lymphokine-activated killer (LAK) cells, which are induced as a consequence of immune reactions, might be immunosuppression.
