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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
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Lymphocytic CD43 and CD45 bear sulfate residues potentially implicated in cell to cell interactions

V Giordanengo1, M Limouse, J F Peyron

  • 1Laboratoire de Virologie, Unité INSERM U364, Faculté de Médecine, Nice, France.

European Journal of Immunology
|January 1, 1995
PubMed

Insights

This study reveals that CD43, a key lymphocyte surface molecule, is sulfated in activated human lymphocytes. Protein sulfation levels influence CD43-mediated cell aggregation, highlighting its role in cell interactions.

Area of Science:

  • Immunology
  • Cell Biology
  • Glycobiology

Background:

  • CD43 is a significant glycosylated molecule on lymphocyte surfaces involved in activation and cell interactions.
  • The precise post-translational modifications and functional roles of CD43, particularly sulfation, require further elucidation.

Purpose of the Study:

  • To investigate the sulfation status of CD43 and CD45 in human activated lymphocytes and CEM T cells.
  • To determine if cell surface protein sulfation modulates CD43-mediated homotypic aggregation.

Main Methods:

  • Utilized 35SO4(2-) incorporation for labeling and visualization of sulfated proteins.
  • Analyzed CD43 and CD45 expression and sulfation in activated lymphocytes and CEM T cells.
  • Assessed the impact of varying sulfation levels on CD43-mediated homotypic aggregation induced by monoclonal antibodies.

Main Results:

  • Demonstrated that CD43 is a sulfated molecule in human activated lymphocytes and CEM T cells.
  • Confirmed that CD45, another lymphocyte surface glycoprotein, is also sulfated.
  • Showed that the extent of cell surface protein sulfation can modulate CD43-mediated homotypic aggregation.

Conclusions:

  • CD43 and CD45 are sulfated glycoproteins on human activated lymphocytes.
  • Protein sulfation plays a regulatory role in CD43-mediated homotypic aggregation.
  • Sulfation sites on CD43 and CD45 may be critical for regulating cell-cell interactions.

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