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Immunoassay of gonadotropins using a fully-automated benchtop analyser
K Kamariah1, J B Lopez, N Satgunasingam
1Division of Radiochemistry, Institute for Medical Research, Falan Pahang, Kuala Lumpur, Malaysia.
Insights
The Abbott IMxTM immunoassay analyser demonstrates excellent analytical performance for total beta human chorionic gonadotropin (Beta hCG), follicle-stimulating hormone (FSH), and luteinising hormone (LH) assays. It offers high precision, strong correlation with established methods, and good sensitivity.
Area of Science:
- Clinical Chemistry
- Immunoassay Technology
- Hormone Analysis
Background:
- The Abbott IMxTM is an automated immunoassay analyzer.
- Accurate measurement of reproductive hormones like Beta hCG, FSH, and LH is crucial for clinical diagnostics.
Purpose of the Study:
- To evaluate the analytical performance of the Abbott IMxTM immunoassay analyser.
- To assess the accuracy, precision, and sensitivity of the IMxTM for total Beta hCG, FSH, and LH assays.
Main Methods:
- Assessed within-run and between-run imprecision (CVs).
- Correlated IMxTM results with radioimmunoassay (RIA) and immunoradiometric assay (IRMA).
- Determined assay sensitivities and evaluated sample carry-over and cross-reactivity.
Main Results:
- Within-run CVs ranged from 2% to 6%; between-run CVs ranged from 4% to 10%.
- High correlation coefficients (r=0.97-0.99) were observed compared to RIA/IRMA.
- Average sensitivities were 3.1, 0.2, and 0.5 IU/L for Beta hCG, FSH, and LH, respectively. No sample carry-over or significant cross-reactivity was detected.
Conclusions:
- The Abbott IMxTM demonstrates reliable analytical performance for Beta hCG, FSH, and LH assays.
- The automated analyzer offers high precision, excellent correlation with reference methods, and good sensitivity.
- The IMxTM is user-friendly, efficient, and suitable for routine laboratory use.
Abstract:
We assessed the analytical performance of the Abbott IMxTM immunoassay analyser for total beta human chorionic gonadotropin (Beta hCG), follicle-stimulating hormone (FSH) and luteinising hormone (LH). The within-run CVs for various analyte concentrations were 2% to 6% while those for between-run imprecision in routine assay ranged from 4% to 10%. IMxTM values correlated well with radioimmunoassay for beta hCG, and immunoradiometric assay for FSH and LH; the correlation coefficients (r) were 0.97, 0.99 and 0.98 for total beta hCG, FSH and LH respectively. The average sensitivities were approximately 3.1, 0.2 and 0.5 iu/l for beta hCG, FSH and LH, respectively. Sample carry-over was not detected and there was negligible cross-reaction between LH and beta hCG in the respective assays. The automatic sample dilution protocol for beta hCG was superior to the manual procedure. The IMxTM is easy to operate and is able to process 24 samples in 40-45 minutes.