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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Protein-protein interactions between human cytomegalovirus IE2-580aa and pUL84 in lytically infected cells

D J Spector1, M J Tevethia

  • 1Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.

Journal of Virology
|November 1, 1994
PubMed

Insights

Human cytomegalovirus immediate-early protein IE2-580aa interacts with a viral protein p75. This study identifies p75 as the product of the UL84 gene, suggesting its role in viral transcription control.

Area of Science:

  • Virology
  • Molecular Biology
  • Gene Regulation

Background:

  • Human cytomegalovirus (HCMV) immediate-early protein IE2-580aa (ppUL122a) regulates viral and cellular gene transcription.
  • IE2-580aa binds the major immediate-early promoter to repress its own transcription.
  • During lytic infection, IE2-580aa interacts with a 75-kDa viral protein (p75), an early protein synthesized at late times.

Purpose of the Study:

  • To identify the viral protein p75.
  • To elucidate the role of p75 in HCMV infection, particularly in relation to IE2-580aa.

Main Methods:

  • Protein identification and characterization.
  • Analysis of viral protein interactions in infected cells.

Main Results:

  • The 75-kDa viral protein (p75) was identified as the product of the UL84 gene.
  • The association between IE2-580aa and pUL84 (p75) was confirmed in infected cells.

Conclusions:

  • The UL84 gene product (pUL84) is a viral protein that interacts with IE2-580aa.
  • The interaction suggests that pUL84 plays a role in the transcriptional control mechanisms of human cytomegalovirus.

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