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Published on: October 19, 2014
Expression of 1D8--a novel non-sialylated B-cell-associated carbohydrate epitope in lymphoproliferative disorders
H Taskov1, M Nikolova, E Dimitrova
1National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
Insights
A novel B-cell epitope, 1D8, is expressed in mature B-cell leukemias and lymphomas. Its expression on activated lymphocytes suggests a role in cell adhesion and migration.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- B-cell malignancies are a diverse group of cancers.
- Understanding novel cell surface markers can aid in diagnosis and treatment.
- The 1D8 epitope is a newly identified B-cell-associated carbohydrate.
Purpose of the Study:
- To investigate the expression of the novel 1D8 epitope in various leukemias and lymphomas.
- To determine if 1D8 expression is lineage- or differentiation-stage restricted.
- To explore the potential function of the 1D8 epitope in lymphocyte activation and behavior.
Main Methods:
- Flow cytometry was used to analyze 1D8 expression.
- 153 cases of leukemias and lymphomas were studied.
- 19 cases of lymphadenopathy served as controls.
Main Results:
- The 1D8 epitope was preferentially detected in mature B-lymphocyte proliferations, including chronic lymphocytic leukemia (CLL) and non-Hodgkin lymphoma (NHL).
- Expression was observed in CD20+ acute lymphoblastic leukemia (ALL).
- Intensive 1D8 expression was noted on activated lymphocytes and some high-grade malignancies, suggesting a link to cellular functional state.
Conclusions:
- The 1D8 epitope is associated with mature B-cell neoplasms.
- Its expression is not strictly lineage- or differentiation-stage restricted.
- Enhanced detection after desialylation suggests the 1D8 epitope may regulate leukocyte adhesion and migration in both normal and malignant cells.
Abstract:
The expression of a novel B-cell-associated carbohydrate epitope (1D8) was studied by means of flow cytometry in 153 well defined cases of leukemias and lymphomas and 19 cases of lymphadenopathy used as controls. The 1D8 epitope was detected preferentially in proliferations of mature B-lymphocytes (11/15 CD20+ acute lymphoblastic leukemia (ALL), 14/16 chronic lymphocytic leukemia (CLL), 4/7 mantle cell non-Hodgkins lymphoma (NHL), 3/8 follicle cell NHL. However its expression did not appear lineage- or differentiation stage-restricted. Intensive expression on in vivo and in vitro-activated lymphocytes as well as in some high grade malignancies indicated a relationship to the functional state of cells. Bearing in mind the enhanced detection of 1D8 upon desialylation, the epitope might be involved in the regulation of adhesion/migration potential of normal leukocytes and their malignant counterparts.
