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Interferon-gamma production of mitogen stimulated peripheral lymphocytes in perinatal cows
H Ishikawa1, T Shirahata, K Hasegawa
1Veterinary Hospital, Hokkaido, Japan.
Insights
Bovine peripheral blood mononuclear cells (PBMC) showed suppressed interferon-gamma (IFN-gamma) production during the perinatal period, with CD4+ T-cells being the major producers. Interleukin 2 (IL-2) supplementation partially restored IFN-gamma levels.
Area of Science:
- Immunology
- Veterinary Science
- Cell Biology
Background:
- Interferon-gamma (IFN-gamma) is a crucial cytokine involved in immune responses.
- The perinatal period in bovines is characterized by significant physiological and immunological changes.
- Understanding immune cell function, particularly T-cell subsets, is vital during this critical phase.
Purpose of the Study:
- To investigate the production of IFN-gamma by bovine peripheral blood mononuclear cells (PBMC) during the perinatal period.
- To identify the specific T-cell subsets responsible for IFN-gamma production.
- To assess the impact of perinatal stress on IFN-gamma production and the potential for modulation with Interleukin 2 (IL-2).
Main Methods:
- PBMC were isolated from bovine peripheral blood during different stages of the perinatal period.
- IFN-gamma activity was measured and neutralized using monoclonal antibodies (MoAb) against bovine IFN-gamma.
- T-cell subsets (CD4+ and CD8+) were analyzed using MoAb, and cell stimulation was performed with concanavalin A (Con A) and phytohemagglutinin (PHA).
- Recombinant human Interleukin 2 (IL-2) was added to cultures to evaluate its effect on IFN-gamma production.
Main Results:
- IFN-gamma production was suppressed in PBMC from the perinatal period compared to non-pregnant controls.
- Monoclonal antibody treatment indicated that CD4+ T-cells were the primary producers of IFN-gamma.
- Concanavalin A-induced IFN-gamma was depressed throughout the perinatal period, while phytohemagglutinin-induced IFN-gamma was markedly depressed around calving.
- Recombinant human IL-2 augmented IFN-gamma production in PBMC from perinatal cows.
Conclusions:
- Bovine CD4+ T-cells are the major source of IFN-gamma during the perinatal period.
- Perinatal stress significantly suppresses IFN-gamma production in response to mitogenic stimulation.
- IL-2 supplementation shows potential for restoring IFN-gamma production in stressed periparturient cattle.
Abstract:
The production of interferon-gamma (IFN-gamma) in bovine peripheral blood mononuclear cells (PBMC) during the perinatal period was studied. IFN-gamma activity was neutralized with monoclonal antibodies (MoAb) to recombinant bovine IFN-gamma. IFN-gamma production was apparently suppressed by treatment of the PBMC with MoAb to bovine pan-T cell. IFN-gamma was more susceptible to treatment with MoAb to CD4+ than that with MoAb to bovine CD8+, suggesting that the CD4+ T-cell subset was the major producer of the lymphokine formed in the culture. IFN-gamma production by concanavalin A (Con A) stimulation was found to be significantly depressed throughout the perinatal period (from 5 weeks before to 7 weeks after parturition) when compared to that of the non-pregnant control group, but phytohemagglutinin (PHA)-induced IFN-gamma production was markedly depressed from 3 weeks before to 2 weeks after calving. The addition of recombinant human interleukin 2 (100 IU/ml) to the PBMC cultures from perinatal cows resulted in the augmentation of IFN-gamma production by stimulation with Con A or PHA.