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CD23 and IgE expression during the human immune response to cutaneous leishmaniasis: possible role in monocyte

I Vouldoukis1, F Issaly, C Fourcade

  • 1Laboratoire de Parasitologie Expérimentale, INSERM U313, Paris.

Research in Immunology
|January 1, 1994
PubMed

Insights

Serum IgE and TNF alpha levels correlate with CD23 expression in Leishmania brasiliensis infection. This immune response marker decreases after successful treatment but persists in therapy-resistant cases, suggesting a role for CD23 in cutaneous leishmaniasis.

Area of Science:

  • Immunology
  • Parasitology
  • Dermatology

Background:

  • Cutaneous leishmaniasis (CL) is caused by Leishmania brasiliensis.
  • Immune responses involving T cells and macrophages are crucial in CL pathogenesis.
  • Serum IgE and Fc epsilon RII/CD23 expression are implicated in inflammatory conditions.

Purpose of the Study:

  • To analyze serum IgE levels and Fc epsilon RII/CD23 expression in L. brasiliensis-infected patients.
  • To correlate these markers with inflammatory mediators like TNF alpha, IL-3, IFN gamma, and IL-4.
  • To investigate the role of CD23 in the immune response to CL before and after therapy.

Main Methods:

  • Serum IgE levels were measured in patients with CL.
  • In situ expression of Fc epsilon RII/CD23, TNF alpha, IL-3, IFN gamma, and IL-4 mRNA was analyzed.
  • Monocytes were purified to assess CD23 function in IgE/anti-IgE-dependent TNF alpha production.
  • Comparisons were made between patients before and after treatment, and with disease-free individuals.

Main Results:

  • Serum IgE and TNF alpha levels increased during L. brasiliensis infection.
  • Elevated CD23, IL-4, and TNF alpha mRNA expression correlated with infection.
  • These markers decreased after successful treatment but remained in therapy-resistant patients.
  • Therapy-resistant patients showed reduced IFN gamma protein expression.
  • CD23 mediates IgE/anti-IgE-dependent TNF alpha production in human monocytes.

Conclusions:

  • Serum IgE and Fc epsilon RII/CD23 expression are associated with active Leishmania brasiliensis infection.
  • CD23 plays a potential role in the acute immune response during human CL.
  • Treatment outcomes in CL may be linked to the modulation of CD23 and IFN gamma expression.

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