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Immunoglobulin-secreting cells in healthy peripheral blood mononuclear cells
Insights
This study quantifies immunoglobulin-secreting cells in peripheral blood mononuclear cells (PBMCs). Results show age-related changes in IgM, IgG, and IgA-secreting cells, potentially impacting in vivo immunity.
Area of Science:
- Immunology
- Cell Biology
Background:
- Immunoglobulin-secreting cells are crucial for adaptive immunity.
- Understanding their prevalence and age-related dynamics is vital for assessing immune function.
Purpose of the Study:
- To quantify the percentage of IgM, IgG, and IgA-secreting cells in healthy adult peripheral blood mononuclear cells (PBMCs) in vitro.
- To investigate the age-related changes in these immunoglobulin-secreting cell populations.
Main Methods:
- Measurement of immunoglobulin-secreting cells (IgM, IgG, IgA) in uncultured PBMCs using in vitro assays.
- Analysis of cell percentages relative to total PBMCs and surface immunoglobulin-bearing cells.
- Comparison of cell numbers across different age groups (childhood vs. adulthood).
Main Results:
- In adult PBMCs, the percentages of IgM-, IgG-, and IgA-secreting cells were 0.053%, 0.099%, and 0.065%, respectively.
- Relative to surface immunoglobulin-bearing cells, these percentages were 0.73% (IgM), 5.2% (IgG), and 3.8% (IgA).
- Immunoglobulin-secreting cell numbers increased with age during childhood but slightly decreased in adults compared to children aged 9-15 years.
Conclusions:
- The study provides baseline data on immunoglobulin-secreting cell frequencies in adult PBMCs.
- Observed age-related decreases in adulthood may contribute to differences in in vivo immune responses.
- These findings offer insights into the maturational changes of B cells and their antibody-secreting capacity.
Abstract:
Immunoglobulin-secreting cells were measured in healthy uncultured peripheral blood mononuclear cells (PBMCs) in vitro. The percentage of IgM-, IgG- and IgA-secreting cells in adult PBMCs was 0.053, 0.099 and 0.065%, respectively. The percentage of IgM-, IgG- and IgA-secreting cells was 0.73, 5.2 and 3.8% of surface IgM-, IgG- and IgA-bearing cells, respectively. The numbers of IgM-, IgG- and IgA-secreting cells increased with age in childhood. However, the numbers of all three classes were slightly decreased in adults compared with children aged 9-15 years. These results may explain the difference in immunity in vivo.